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孤雌生殖海带对草丁膦的敏感性
吴韵, 邹立红, 姜鹏, 孙国琼, 秦松, 曾呈奎
中国科学院海洋研究所 青岛266071
摘要:
于1999年4–7月，以孤雌生殖海带为材料，运用统计学方法研究其对草丁膦的敏感性，以期得到一定剂量和时间范围内草丁膦对不同长度孤雌生殖海带的半数致死剂量(LD50)。结果表明，孤雌海带在0.5–1.6cm长度范围内，草丁膦的半数致死剂量与海带的长度不相关，而与草丁膦的剂量和作用时间相关。孤雌生殖海带对草丁膦比对氯霉素和潮霉素更为敏感，提示bar基因有可能成为海带基因工程更为理想的选择标记基因。
关键词:  海带  草丁膦  选择标记
DOI：
分类号:
基金项目:中国科学院生物科学与技术研究特别支持费资助项目，STZ97-3-09号
SENSITIVITY OF PARTHENOGENETIC SPOROPHYTES OF LAMINARIA JAPONICA PHAEOPHYTA TO BASTA
WU Yun, ZOU Li-Hong, JIANG Peng, SUN Guo-Qiong, QIN Song, ZENG Cheng-Kui
Institute of Oceanology,The Chinese Academy of Sciences,Qingdao,266071
Abstract:
In the experiment, young sporophytes of parthenogenetic Laminaria japonica were divided into three groups according to their total lengths: a. (5.00±0.06)mm, b. (9.50±0.10)mm and c. (16.10±0.13)mm, and were treated with 13 concentrations of basta solution in each group respectively. Basta dose are 0–21.2μg/ml. LD50 at 36, 48, 60 and 72 hours were calculated. Results showed that LD50 of sprorphytes of parthenogenetic L. japonica (0.5–1.6)cm to basta is not correlated with its length. The sporophytes of parthenogenetic L. japonica are more sensitive to basta than to chloramphenicol and hygromycin that have been used as selection pressure in genetic engineering of L. japonica. Our observation suggests that bar gene which encodes phosphinothricin-N-acetyltrensferase can be a more efficient selectable marker gene.
Key words:  Laminaria japonica, Basta, Selectable marker
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