| 摘要: |
| 于1994年9月–1995年12月，在青岛近海采集37尾鲈鱼的生化样品，采用淀粉胶和聚丙烯酰胺凝胶2种电泳方法，分析了LDH、MDH、MEP、IDHP、PGM、G3PDH、AK、CK、ADH、SDH、G6PDH、GDH、PGDH、CAT、MPI、EST、SOD等同工酶在鲈鱼眼睛、肌肉、心脏、肝脏、肾脏、鳃、胸鳍等7种组织或器官中的表达情况，并对同工酶表型进行了生化遗传分析，以期为其种质资源保护和开发，以及遗传育种等方面的研究提供基础资料。结果表明，17种同工酶由31个基因座位编码，其中Mep-1、Pgm-1、Sdh、Gdh、Pgdh、Mpi、Est-1、Est-4等8个基因座位为多态。多态座位比例为25.8%。 |
| 关键词: 鲈鱼 同工酶 生化遗传分析 |
| DOI： |
| 分类号: |
| 基金项目:国家攀登计划B资助项目，PDB6-5-2号 |
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| BIOCHEMICAL GENETICS OF LATEOLABRAX JAPONICUS POPULATION Ⅰ. BIOCHEMICAL GENETIC ANALYSIS OF ISOZYMES |
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XU Cheng, WANG Ke-Ling, YOU Feng, WU Su-Qi, ZHANG Pei-Jun
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Institute of Oceanology,The Chinese Academy of Sciences,Qingdao,266071
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| Abstract: |
| During Sep. 1994–Dec. 1995, 37 sea bass (Lateolabrax japonicus) samples were collected from coastal water of Qingdao, Shandong Province. Starch gel electrophoresis and polyacrylarnide gel electrophoresis were used to detect expression of isozymes in sever organs or tissues: eye, muscle, heart, liver, kidney, gill and pectoral fin. LDH, MDH, MEP, IDHP, PGM, G3PDH, AK, CK were analyzed in Tris-citric acid buffer system (TC, pH=6.9); ADH, SDH, G6PDH, GDH, PGDH, CAT and MPI were analyzed in EDTA-boric acid-Tris buffer system (EBT, pH =8.9); SOD and EST in Tris-gly buffer system (TG, pH =8.3). Biochemical genetic analysis showed 17 isozymes were coded by 31 gene loci, 8 of which were found polymorphic. Of the polymorphic loci, Mep-1, Gdh, Pgdh, Est-1 and Est-4 each had two alleles and three phenotypes; Pgm-1, Sdh and Mpi each had three alleles, all six phenotypes of Mpi were observed, but only four of Pgm-1 and three of Sdh were observed, low frequency pheotypes were absent. Mean proportion of polymorphic loci was 25.8%. |
| Key words: Lateolabrax japonicus, Isozyme, Biochemical genetic analysis |
