| 摘要: |
| 于1997年10月在厦门西海域采集纤毛虫常见种—具沟急游虫，采用改进的荧光标记法在实验室条件下进行纤毛虫的摄食速率研究。结果表明，改进的荧光标记法具有操作简便、节省昂贵的荧光染料、固定液浓度易于掌握等优点。用该方法在常温下(22℃)测得纤毛虫对细菌和微藻的摄食速率分别为4.224pgC/(cell*h)和5.0pgC/(cell*h)。将此实验结果外推至自然海区，可得台湾海峡南部夏季纤毛虫对细菌、微藻的同化率分别为0.0176mgC/(m3*d)和0.0201mgC/( m3*d);北部冬季分别是0.0238mgC/( m3*d)和0.0272mgC/( m3*d)。在实验条件下，温度较低时(14–26℃)对纤毛虫的摄食速率影响较大，但在较高温度(26–34℃)时差异不明显。 |
| 关键词: 荧光标记技术 纤毛虫 具沟急游虫 摄食速率 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目，49636220号，49776308号 |
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| THE GRAZING RATE OF STROMBIDIUM SUCALTUM USING A MODIFIED FLUORESCENCE-LABELED TECHNIQUE |
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HONG Hua Sheng, KE Lin, HUANG Bang Qin, LIN Xue Ju
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Environmental Science Research Center, Xiamen University, Ministry of Education, Marine Environmental Laboratory, Xiamen University, Xiamen, 361005
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| Abstract: |
| Studies on the grazing rate of Strombidium sucaltum, one of the common species of ciliates in Western Sea, Xiamen, were carried out under laboratory conditions using a modified technique of fluorescence-labeled bacteria or nanophytoplankton, in Oct. 1997. Compared to the previous fluorescence-labeled technique, two steps were changed. Firstly, ciliates collected in the field were cultured under laboratory conditions prior to the grazing experiments, in order to obtain larger amount of ciliates. Secondly, the filtering step was replaced by pipetting, so that individual ciliate could be more easily identified. These changes have the following advantage: the procedures are simplified because ciliate staining is not required and the concentration of fixative can be adjusted. The grazing rates of Strombidium sucaltum on bacteria and nanophytoplankton under room temperature (22°C) were 4.224pgC/(cell*h) and 5.0pgC/(cell*h) , respectively. Extrapolating the results to in situ situations, the assimilation rates of carbon by ciliates on bacteria and nanophytoplankton in the southern Taiwan Strait in summer were 0.0176mgC/(m3*d) and 0.0201mgC/(m3*d), respectively, and in the northern Taiwan Strait in winter were 0.0238mgC/(m3*d) and 0.0272mgC/(m3*d), respectively. An increase in temperature led to an increase in grazing rate of ciliate over the range of 14–26°C while at higher degrees (26–34°C) no further increase in grazing rate was observed. |
| Key words: Fluorescence- labeled technique, Ciliate, Strombidium sucaltum, Grazing rate |
