| 摘要: |
| 研究了条斑紫菜不同栽培品系间在单孢子形成方面的差异,并采用RAPD方法研究了它们之间的遗传距离,还研究了温度、藻体不同发育时期、干出对单孢子形成的影响。结果表明,产生单孢子多的品系与单孢子少的品系间遗传距离较远,单孢子多的品系间、单孢子少的品系间遗传距离都较近;室内培养环境能够形成单孢子的最低水温高于自然海区;大于几十个细胞的藻体在合适的温度下可以持续形成单孢子,一直到藻体成熟;在成熟的藻体上仍有单孢子放散,但大量的果胞也可以以与单孢子相同的方式放散并萌发成苗。干出对单孢子的产生没有明显的影响。 |
| 关键词: 条斑紫菜 单孢子 栽培品系 干出 RAPD |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金农业倾斜项目资助,39770593号 ;国家海洋863资助项目,819-03-06号 |
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| STUDY ON MONOSPORE PRODUCTION OF PORPHYRA YEZOENSIS |
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MEI Jun-Xue1, FEI Xiu-Geng2, WANG Bin3
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1.Marine Biotechnology Department of Shandong University Weihai Branch,Weihai,264200;2.Institute of Oceanology,The Chinese Academy of Sciences,Qingdao 266071;3.Institute of Genetics,The Chinese Academy of Sciences,Beijing, 100101
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| Abstract: |
| The paper studied the difference in monospore production in 4 cultivated strains of Porphyra yezoensis and their genetic distance with RAPDs analysis. The results showed that the genetic distance between the strain that can produce few monospores and the strain that can produce a mass of monospores farther than both the distance between few-monospore strains and the distance between mass-monospore strains. The effects of temperature, the developmental phases of the thalli and out- drying on monospores production were also studied. The lowest temperature for monospore formation in laboratory was different from that in the sea (they are 12°C and 7–8°C , respectively). After the initiation of forming monospores when they were about 0.2mm long, the leafy thalli produced monospores all through their life in suitable temperature (17°C). Carpogonia on the edge of the mature leafy thallus could be discharged and geminate in the same way as monospores did. Out- drying did not appear to have notable effect on monospore production. |
| Key words: Porphyra yezoensis, Monospores, Cultivated strains, Out- drying, RAPD |
