| 摘要: |
| 于1998年9月在青岛鲁迅公园附近礁石区采集紫贻贝(Mytilus edulis),采用实验室培养的方法,初步研究了塔玛亚历山大藻(Alexandrium tamarense,ATHK)产生的麻痹性贝毒(Paralytic Shellfish Poison,PSP)在其体内累积、转化与排出的规律。结果表明,在累积实验阶段,紫贻贝内脏和肌肉中的PSP毒素含量均随实验时间的延长而逐渐增加,累积实验结束时,平均每只贝体内的PSP毒素含量为13.40nmol,毒性水平为12.24μgSTXEq/100g。紫贻贝内脏中的毒素含量远远高于肌肉,内脏中PSP毒素占贝体内PSP毒素总量的97.5%。在8天的排出实验阶段,贝体内的PSP毒素总量呈下降趋势,实验结束时,PSP毒素共排除了约50%,每天排除率约为9%。 |
| 关键词: 塔玛亚历山大藻 麻痹性贝毒 紫贻贝 累积 排出 转化 |
| DOI： |
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| 基金项目:国家自然科学基金重大资助项目,39790110号; 国家自然科学基金资助项目,49576301号、49906007号; 国家自然科学基金主任基金资助项目, 39950001号; 山东省科委资助项目“鲜活贝类卫生优质调控机理研究”; 中国科学院知识创新工程资助项目,KZCX2- 206号 |
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| ACCUMULATION, TRANSFORMATION AND ELIMINATION OF PSP IN MYTILUS EDULIS |
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YAN Tian, FU Meng, LI Jun, YU Ren-Cheng, ZHOU Ming-Jiang
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Institute of Oceanology,The Chinese Academy of Sciences,Qingdao 266071
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| Abstract: |
| Using a PSP (Paralytic Shellfish Poisoning) producing strain Alexandrium tamarense (ATHK), we studied toxin accumulation, transformation and elimination of PSP in mussels Mytilus edulis. The rsults show that the PSP content increased with time both in visceral and muscle during an 8-day accumulation period when mussels were feeding with A. tamarense. At the end of the accumulation period, the mean toxin amount and accumulation efficiency in each mussel were 13.40nmol, 12.45% (comparing to the total PSP amount from algae), respectively. The toxicity level was 12.24μgSTX Eq/100g, which did not reach the sanitation standard (80μgSTX Eq/100g). The accumulation of visceral was more intense than that of muscles. The toxin amount in visceral of average each mussel was 13.07nmol, with its accumulation efficiency being 12.14% at the end of accumulation period, whilst those of muscles were only 0.33nmol and 0.31%, respectively. The percentage of toxin accumulated in visceral was 97.5% of total, indicating that visceral was the main part for toxin accumulation in shellfish. There are also different toxin profiles of the alga and mussel, indicating that the toxins may have transformed in mussels. During the eight-day elimination period, the amount of total PSP decreased with an elimination rate of about 9% per day. Except GTX1, 4, the main PSP composition in muscle which decreased too, GTX2, GTX3, NEO and dsSTX did show decrease, but STX increased during the elimination period. This was probably caused by complicated transformation among PSP compositions. Further studies will focus on PSP depuration of shellfish to provide techniques for developing sanitary shellfish industry in China. |
| Key words: Alexandrium tamarense, PSP, Mytilus edulis, Accumulation, Elimination, Transformation |
