| 摘要: |
| 1996年6月初,对福建省平潭县真鲷网箱养殖场发生的弧菌病进行研究,并确定该病病原菌为最小弧菌(Vibrio mimicus)。采用硫酸铵沉淀、DEAE-纤维素层析及Sephadex-G100层析等方法对最小弧菌产生的外毒素进行研究。结果表明,该外毒素为单一的多肽,分子量为30.456kD,对真鲷的LD50为4.48μg,对真鲷等养殖动物的溶血性最强,对Vero细胞的CD50值为0.48μg,肠毒性弱阳性。该外毒素与最小弧菌产生的其他毒力因子相比,性质独特,是一类新的毒力因子,建议命名为Vm-Pm毒素。
|
| 关键词: 真鲷,最小弧菌,外毒素Vm-Pm |
| DOI：10.11693/hyhz200201013013 |
| 分类号: |
| 基金项目:中国科学院“九五”重点支持项目资助,KZ952-S1-415号 |
|
| PURIFICATION AND CHARACTERIZATION OF THE EXOTOXIN Vm-Pm PRODUCED BY VIBRIO MIMICUS |
|
WU Hou-Bo, PAN Jin-Pei
|
|
South China Sea Institute of Oceanology,The Chinese Academy of Sciences
|
| Abstract: |
| An extracellular toxin Vm-Pm produced by Vibrio mimicus was purified by the procedures of salting out of ammonium sulfate, anion exchange chromatography on DEAE-cellulose and gel filtration chromatography of Sephadex G100. Toxin Vm-Pm is a polypeptide with a molecular weight of 30.456kD, as determined by SDS polyacrilamide gel electrophoresis.
Pathogenicity of the heat-labile toxin Vm-Pm for red sea bream was evaluated. The toxin was quite pathogenic to red sea bream, and the LD50 for red sea bream was 4.48μg. The toxin showed remarkable hemolytic activities for red blood cells from red sea bream, crucian carp, human and some other animals. The toxin exhibited a postive cytotoxic response for Vero cells and the CD50 for Vero cell is 0.48μg. The enterotoxic activities of the toxin for rabbit is weak; it could cause only a little fluid accumulation in ligated rabbit ileal loops.
Compared with other known toxic factors produced by Vibrio mimicus, toxin Vm-Pm shows unique characteristics. It is a new toxic factor produced by Vibrio mimicus, therefore the authors propose to name the toxin“Vm-Pm”toxin.
|
| Key words: Red sea bream (Pagrus major),Vibrio mimicus, Extracellular toxin Vm-Pm |
