| 摘要: |
| 利用聚丙烯酰胺垂直板电泳技术对中国栉孔扇贝和日本栉孔扇贝两个自然种群的6 种同工酶(MDH、ADH、G- 6- PDH、IDH、GDH、ME) 进行了研究, 得到了基本酶谱, 对其进行了生化遗传分析。共记录了18 个座位28 个等位基因并对其差异进行了比较和讨论。两种群各自具有种群内的个体差异及特征谱带, 可将其作为栉孔扇贝亚种间差异的一种分子标记, 并为系统分类及贝类学基础研究提供一定的参考数据。 |
| 关键词: 栉孔扇贝, 同工酶, 生化遗传分析 |
| DOI： |
| 分类号: |
| 基金项目:国家"973"资助项目"扇贝生物种群遗传结构及遗传多样性的应用", G1999012008 号 |
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| BIOCHEMICAL GENETIC ANALYSIS OF SIX ISOZYMES ON CHLAMYS FARRERI |
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WANG Zhi-Zheng1, LI Tai-Wu2, LIU Yan3, SUN Xiu-Qin4, GUO Hao5, LI Chun-Mao6, SONG Lin-Sheng7, XIANG Jian-Hai7
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1.Zhejiang Ocean University;2.Life Science and Biotechnology School , Ningbo University;3.Life Science School , Liaoning Normal University;4.First Institute of Oceanography, State Oceanic Administration;5.Institiute of Environment Protection of Oceanography, State Oceanic Administration;6.Institute of Changhai Aquaculture;7.Institute of Oceanology, The Chinese Academy of Sciences
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| Abstract: |
| Six isozymes (MDH, ADH, G-6-PDH, IDH, GDH, ME) in adductor muscle of scallop( Chlamys farreri Jones et Preston) in two populations were analyzed by vertical polyacrylamide gel electrophoresis( PAGE) . The live materials were collected from the natural seawaters of Dalian, China and Japan from 1998 to April, 1999. Dissected the samples, fetched the adductor muscle and stored them at - 70℃. By homogenizing in ice and centrifuging at 4℃, 13000r/min, the supernatant of samples were extracted and stored at - 20℃ for electrophoresis( buffer system: Tris-Gly, pH= 8.3; voltage: 300V 40—50min in concentrated gel( T = 3.6%, pH= 6.7) , 200V 4 hours in separated gel(T= 8.2%, pH= 8.9) ) . The results imply the biochemical genetic traits of 6 isozymes, including their locus number, structure, alleles etc. On these basis, the basic electrophoretic patterns of the 6 isozymes were gained. In two populations, altogether 18 gene loci and 28 alleles were recorded, among which the expression of 8 loci including Idh-1, Idh-2, Adh-1, Adh-2, Me-1, Me-3, G-6-pdh-3 and Mdh-3 were identical in all samples, the rest were different at different levels. At the same time, the individual-difference existed in each population. This paper compared and discussed the result. Some bands only expressed in specific population, they can be used as effective genetic markers for all kinds of studies. |
| Key words: Chlamys farreri, Isozyme, Biochemical genetic analysis |
