| 摘要: |
| 对栉孔扇贝分别注射硒化卡拉胶和酵母葡聚糖后, 采用生化方法, 于1h、15h 和30h分别测定栉孔扇贝血清和血细胞中2 种参与免疫防御的水解酶酸性磷酸酶( ACP) 和溶菌酶的活力。结果表明, 注射硒化卡拉胶后, 栉孔扇贝血清和血细胞中ACP 活力在1h 和30h 时均为实验组显著高于对照组, 溶菌酶活力也均在1 和30h 时实验组显著高于对照组。注射酵母葡聚糖后, ACP 活力在1h、15h 和30h 时均为实验组极显著地高于对照组, 溶菌酶活力则均为实验组与对照组差异不显著。说明硒化卡拉胶对栉孔扇贝血淋巴中ACP 和溶菌酶的活力均有增强作用, 而酵母葡聚糖仅对其ACP 活力有增强作用。 |
| 关键词: 硒化卡拉胶, 酵母葡聚糖, 栉孔扇贝, 血淋巴, 水解酶 |
| DOI： |
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| 基金项目:国家"973"课题资助项目, G1999012005 号 |
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| THE EFFECTS OF SELENO-CARRAGENNAN AND β-1, 3-GLUCAN ON THE ACTIVITIES OF HYDROLYTIC ENZYMES IN THE HAEMOL YMPH OF CHLAMYS FARRERI |
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SUN Hu-Shan1, LI Guang-You2
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1.Biological Science and Technology Department, Yantai Normal College;2.Key Lab of Marine Bioactive Substances, State Oceanic Administration
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| Abstract: |
| Two kinds of hydrolytic enzymes to participate in the immune defense in the haemolymph of Chlamys farreri were assayed at 1h, 15h and 30h after injection with seleno-carragennan or β-1, 3-glucan. After injection with seleno-carragennan, the acid phosphatase (ACP) activities of experimental groups in serum and haemocytes were much higher than that of control groups at 1 and 30h; the differences of lysozymal activities of experimental and control groups in serum and haemocytes were significant except the group in serum after 15h. After injection with β-1, 3-glucan, the ACP activities of experimental groups in serum and haemocytes were much higher than that of control groups at 1h, 15h and 30h; the differences of lysozymal activities of experimental and control groups both in serum and haemocytes were not significant at 1h, 15h and 30h. It was suggested that the Seleno-carragennan could enhance the activities of ACP and lysozyme in serum and haemocytes of Chlamys farreri, but the β-1, 3-glucan could only enhance the activities of ACP. |
| Key words: Seleno-carragennan, β-1, 3-glucan, Chlamys farreri, Haemolymph, Hydrolytic enzyme |
