| 摘要: |
| 分属绿藻门、红藻门、褐藻门的海藻样品于2000 年5 月采于山东青岛和威海附近沿海, 将海藻样品的乙醇提取物进行薄层层析分离和抗大肠杆菌、金黄色葡萄球菌活性测定。结果表明, 抗大肠杆菌活性较强的海藻样品有: 鸭毛藻、酸藻、松节藻和孔石莼的水相; 抗金黄色葡萄球菌活性较强的提取物有: 鸭毛藻、海黍子、松节藻、小粘膜藻的乙酸乙酯相以及酸藻的水相。从不同门类海藻的抗菌活性看, 褐藻最强, 红藻次之, 而绿藻活性最弱。薄层层析分离得到16 个层析带具有抗金黄色葡萄球菌活性, 11 个层析带具有的抗大肠杆菌性。其中褐藻门的酸藻、海黍子的3 个层析带具有很强的抗金黄色葡萄球菌活性, 酸藻的2 个层析带也能强烈地抑制大肠杆菌的生长。在同样的实验条件下, 对常见抗生素进行的对照实验表明,这5 条层析带的活性比常见的抗生素高。 |
| 关键词: 海藻, 抗菌活性, 薄层层析(TLC) |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金重点项目, 29932030 号; 山东省自然科学基金项目,Q99E07 号 |
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| STUDY ON THE ANTIBACTERIAL ACTIVITIES OF THE ETHANOL EXTRACTS OF SEAWEED |
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XU Nian-Jun, FAN Xiao, HAN Li-Jun, LIN Wei, ZENG Cheng-Kui( C.K. Tseng)
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Institute of Oceanology, The Chinese Academy of Sciences
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| Abstract: |
| The ethanol extracts of seaweed belonging to Chlorophyta, Rhodophyta and Phaeophyta, collected from Shandong coast in May 2000, were assayed their activities against two kinds of bacteria of Escherichia coli and Staphylococcus aurens. As a result, the water phase of extracts of Symphyocladia latiuscula, Desmarestia viridis, Rhodomela confervoides and Ulva pertusa showed activities against Escherichia coli.The EtOAc phase of extracts of Symphyocladia latiuscula, Sargassum kjelimanianum, Rhodomela confervoides, Leathesia difformes and the water phase of extract of Desmarestia viridis showed activities against Staphylococcus aurens . Brown algae had the highest activities, then red algae, and green algae had the lowest activities. Five species of seaweed, Desmarestia viridis, Sargassum kjelimanianum, Leathesia difformes, Symphyocladia latiuscula and Rhodomela confervoides showed high antibacterial activities. Thin layer chromatography(TLC) was used to separate the extracts of the five species of seaweed and each band was examined for their anti-bacterial activities. It turned out that 16 bands showed activities against Staphylococcus aurens and 11 bands showed activities against Escherichia coli. Three bands from Desmarestia viridis and Sargassum kjelimanianum had powerful activities against Staphylococcus aurens and two bands from Desmarestia viridis could inhibit the growth of Escherichia coli vigorly. Compared with some common antibiotics, the activities of these five bands from Desmarestia viridis and Sargassum kjelimanianum were stronger. |
| Key words: Seaweed, Antibacterial activity, Thin layer chromatography(TLC) |
