| 摘要: |
| 分属绿藻门、红藻门、褐藻门的海藻样品于2000年采于山东青岛和威海沿海,首次采用人白细胞弹性蛋白酶体外筛选技术对39种海藻样品的甲醇提取物进行了抗炎活性筛选,以期从海藻中寻找天然抗类风湿性关节炎等炎症性疾病的药物活性前体。结果表明,18种海藻(7种褐藻,11种红藻)对HLE有抑制活性,而绿藻缺乏抗HLE活性。这18种海藻中,7种表现出较强的抑制活性,分别为:鸭毛藻、海头红、江蓠、细枝软骨藻、粗枝软骨藻、小粘膜藻和绳藻,其中尤以绳藻的抑制活性最强(在浓度为25μg/ml时可达98.9%)。
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| 关键词: 海藻,筛选,抗炎,人白细胞弹性蛋白酶 |
| DOI：10.11693/hyhz200302004004 |
| 分类号: |
| 基金项目:国家863计划资助项目,2001AA620403号、2001AA620503号、国家自然科学基金资助项目,29932030号 |
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| A SCREENING FOR THE ANTI-INFLAMMATORY EFFECT OF ALGAL EXTRACTS |
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NIU Rong-Li, FAN Xiao, HAN Li-Jun
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Institute of Oceanology, The Chinese Academy of Sciences
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| Abstract: |
| Of many methods adopted to develop new drugs from natural products against different pathology mechanisms of inflammatory process, we are probably the first to make an attempt to investigate natural substances from marine organisms against human leukocyte elastase (HLE, EC3.4.21.37) in vitro. In our investigation, the methanol extracts of thirty-nine marine algae (5 green, 13 brown and 21 red) collected from the coasts of China were evaluated for the anti-inflammatory activity. Significant biological activity was obtained in seven algae: Symphyocladia latiuscula, Plocamium telfairiae, Gracilaria verrucosa, Chondria tenuissima and Chondria crassicaulis. And the most potential species was Chorda filum with 98.9% inhibition at 25μg/ml, which shows that Chorda filum may have some compounds with remarkable inhibitory effect against HLE. Differently, green algae have no activity in the experiment. In conclusion, our research demonstrates that marine algae are a potential source
of natural products showing anti-inflammatory effect against HLE and merit further investigation.
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| Key words: Algae, Screening, Anti-inflammatory, Human leukocyte elastase |
