| 摘要: |
| 采用测定净光合放氧速率的方法研究了温度、光照强度和pH对4株雨生红球藻光合作用和生长繁殖速率的影响。结果表明, 温度、光照强度和pH对雨生红球藻的光合作用和生长速率的影响十分显著；4株雨生红球藻光合作用的最适温度、光饱和点和pH分别为: Haematococcus pluvialis 26: 15℃, 250μmol/(m2·s) 和 pH 7.0; Haematococcus pluvialis 30: 20℃, 200μmol/(m2·s)和 pH8.0; Haematococcus pluvialis 34: 15℃, 200μmol/(m2·s) 和 pH7.0; Haematococcus pluvialis WZ: 10℃, 140μmol/(m2·s)和 pH6.0。雨生红球藻细胞数量的增加与光照时间成正比。初步认为H. pluvialis 30、H. pluvialis 26 和 H. pluvialis WZ 是3个有潜力适合于大规模生产的藻种。
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| 关键词: 雨生红球藻,光合作用,生长速率,温度,光照强度,pH |
| DOI：10.11693/hyhz200305011011 |
| 分类号: |
| 基金项目:国家自然科学基金重大项目“中国孢子植物志,团藻目(I)”资助,CNSF39899400号;中国科学院武汉植物研究所所长基金资助项目“我国红球藻资源收集、藻种分离纯化和培养条件研究”,04015107号 |
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| EFFECTS OF TEMPERATURE, LIGHT INTENSITY AND PH ON PHOTOSYNTHESIS AND GROWTH RATE OF HAEMATOCOCCUS PLUVIALIS |
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ZHANG Bao-Yu, LI Ye-Guang, LI Zhong-Kui, GENG Ya-Hong, HU Hong-Jun
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Wuhan Institute of Botany, The Chinese Academy of Science
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| Abstract: |
| The effects of temperature, light intensity and pH on photosynthesis and growth rate of four Haematococcus pluvialis strains were investigated. Results showed that photosynthesis and growth rate were significantly affected by temperature, light intensity and pH. Optimal temperature, saturation light intensity and pH value of four Haematococcus strains were as follows: Haematococcus pluvialis 26: 15℃, 250μmol/(m2·s) and pH7.0; Haematococcus pluvialis 30: 20℃, 200μmol/(m2·s) and pH8.0; Haematococcus pluvialis 34: 15℃, 200μmol/(m2·s) and pH7.0; Haematococcus pluvialis WZ: 10℃, 140μmol/(m2·s) and pH6.0. Optimal cultivation temperature were 20℃, 25℃, 20℃, 15℃, respectively. Growth rate of H. pluvialis was increased by prolongation of photophase and the increase of cell number was in direct proportion to the length of photophase. H. pluvialis WZ was especially adapted to low temperature and H. pluvialis 26, H. pluvialis 30, H. pluvialis WZ were considered as the potential strains for mass culture according to their photosynthetic characteristics and adaptation to temperature.
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| Key words: Haematococcus, Photosynthesis, Growth rate, Temperature, Light intensity, pH |
