| 摘要: |
| 采用光镜和电镜方法，观察了吊笼式人工养殖三倍体皱纹盘鲍性腺发育情况，并与二倍体进行比较。结果表明，三倍体鲍性腺发育分期基本与二倍体相同，但雄性腺发育停止在生长期，雌性腺少数可达成熟期，没有发现排放期。三倍体鲍性腺发育较二倍体差，表观看其性腺薄，只包围肝胰腺1／3—1／2，颜色浅。 7一l0月间检查的86个二倍体个体中成熟期的占24．4％ ，休止期占11．6％。而83个三倍体个体中，成熟期占1．2％，休止期占72．3％。三倍体成熟卵母细咆直径、核径、线粒体直径、胶膜厚度较二倍体大，但卵黄颗粒、线粒体、粗面内质网等细胞器较二倍体 少。 |
| 关键词: 三倍体皱纹盘鲍, 性腺发育, 生物学 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目，39870700号 |
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| THE BIOLOGY OF GONADIAL DEVELOPMENT OF TRIPLOIDY ABALONE(HALIOTIS DISCUS HANNAI) |
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LI Xia1, YAN Song1, ZHANG Guo-Fan2, WANG Zi-Chen1
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1.Key Lab. of Mariculture & Biotechnology Agriculture Ministry，Dalian Fisheries University;2.Institute of Oceanology , Chinese Acadmey of Sciences
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| Abstract: |
| Contrary to most previous research, which focused on methods of inducing triploid, growth and hereditary features of abalone(Haliotis discus Hannai), this work investigated gonadal development of triploid and diploid disk abalone cultured artificially in cages with light microscopy and electromicrocopy. During 1997, triploid disk abalones were induced by caffeine and heat shock. The results showed that gonadal development stages of the triploid abalones were the same as that of the diploid, but testis development stopped during the growth period while the ovary developed to the mature stage. The spawning stage was not found. The triploid gonad was thin and light in colour, the length was only about the 1/3— 1/2 length of the pancreance. The percentage of mature indjvidua1s were 24.4% and 11.6% in the resting period in diploid abalones, but 1.2% in the mature stage and 72.3% in the resting stage in triploid abalones. Gonad development of triploid was prevented. The diameter of the oocyte, nuclei, mitochondria of triploid abalone were longer than that of the diploid as was the jelly coat. The mount of yolk granula, however, the mitochondria and the endoplasmic reticulum of the oocyte in the triploidy gonad were less than that of the diploid. This may influence the development of early life and result in larvae death. The structure of spermatic and spermiogenesis were observed in the dip1oid of abalones but no mature sperm in the testis of the triploid abalone was found. There was also a lot of deformed spermatid with abnormal cell organs, such as the mount of mitochondria and endoplasmic reticulum decreased and the lysosome increased and muhip1e holes in the nuclei. In addition, the mitochondria was not arranged in a rose-like, style which, while distinct from Crassostrea gigas Thunberg and Pinctada martensii(Dunker), was common to that found in Saccostrea commercialis, Mya arenaria and Haliotis diversicolors. |
| Key words: Triploidy abalone, Gonad development, Biology |
