| 摘要: |
| 采用生态学单因子梯度试验方法,研究了水环境中添加0.01、0.10、1.00、5.00mg/L的铜(Cu2+)对中华绒螯蟹肝胰腺、血淋巴和鳃代谢酶活力的影响。结果表明,随着Cu2+浓度的增高,肝胰腺和血淋巴的铜锌超氧化物歧化酶(Cu-Zn SOD)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活力和血蓝蛋白均呈先增加后减少的变化趋势。鳃钠钾三磷酸腺苷酶(Na+,K+-ATPase)、钙镁三磷酸腺苷酶(Ca2+,Mg2+-ATPase)和谷丙转氨酶(GPT)活力均随着Cu2+浓度的增高而降低。随着Cu2+浓度的增高,肝胰腺髓性过氧化物酶(MPO)活力呈先减少后增加的变化趋势。水环境中添加0.01mg/L Cu2+就可对中华绒螯蟹代谢酶产生显著影响,并随着Cu2+浓度的增高,对中华绒螯蟹机体的影响逐渐加剧。表明代谢酶活力的变化可以灵敏地反映Cu2+的胁迫程度和毒性。 |
| 关键词: 中华绒螯蟹,肝胰腺,血淋巴,鳃,代谢酶,铜 |
| DOI： |
| 分类号: |
| 基金项目:中国水产科学研究院首席科学家资助金项目,1-105011号 |
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| EFFECTS OF WATER-BORNE COPPER ON ACTIVITIES OF METABOLISM ENZYMES IN ERIOCHEIR SINENSIS |
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YANG Zhi-Biao1, ZHAO Yun-Long1, ZHOU Zhong-Liang1, LI Na1, YANG Jian2
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1.School of Life Science, East China Normal University, Shanghai, 200062;2.Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081
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| Abstract: |
| Effect of water-borne copper Cu2+ in different concentrations (0.00, 0.01, 0.10, 1.00, 5.00mg /L) on activities of several main metabolic enzymes in hepatopancreas, hemolymph and gill of Eriocheir sinensis were studied by Single Gradient Factor Experiments. The result showed early rise-up and later fall-down curve in Cu-Zn SOD activity in hepatopancreas, with increasing of Cu2+ level and the turn-over point of 26.018U/mg at 1.00mg /L Cu2+. The maximum activities of ACP (170.861U/mg) and AKP (267.238U/mg) were observed at 0.10mg/L Cu2+, while the minimum activities of ACP (123.879U/mg) and AKP (133.244U/mg) were at 5.00mg/L Cu2+. The activity of MPO was significantly different between the controls and treated groups when Cu2+ level rises. The lowest (1.605U/g) and highest value (8.743U/g) of the enzyme were at the water-borne of 0.01mg/L Cu2+ and 5.00mg/L Cu2+, respectively. Gill impairment by Na+, K+-ATPase and Ca2+, Mg2+-ATPase activities occurred when exposed to water-borne Cu2+, obtaining the highest and lowest value in the controls and 5.00mg/L Cu2+ treated group, respectively. In hemolymph the activity of Cu-Zn SOD increased in the beginning and then decreased when Cu2+ concentration increased, highest (229.047U/ml) activities at 1.00mg/L Cu2+ and lowest (199.539U/ml) at 5.00mg/L Cu2+. The highest activity of GPT (38.132U/L) was obtained in the controls, while the lowest one (0.024U/L) was observed at 5.00mg/L Cu2+ group. Increasing Cu2+ level was also associated with the activity of ACP, showing early increase at the peak of 3.529U/100ml and later decrease being at the trough of 2.171U/100ml corresponding to 0.10 and 5.00mg/L Cu2+, respectively. Similarly the activity of AKP peaked to 2.496U/100ml at 0.10mg/L Cu2+ and dipped to 1.642U/100ml at 1.00mg/L Cu2+. In addition, hemocyanin activity in the hemolymph exhibited a trend of early up and later down, 485.227U/L the maximum at 0.10mg/L Cu2+ and the minimum 89.200U/L at 5.00mg/L Cu2+.
It should be emphasized that a significant impairment to metabolism enzymes can occur even at 0.01mg/L Cu2+, a very low level that is in the range of National Water Quality Standard for Fisheries (GB11607-89). The changes in the activities of enzyme can be used as a sensitive biomarker to Cu2+ toxicity for E. sinensis. More species-specified national water quality standards are suggested to be applied to different economic animals. |
| Key words: Eriocheir sinensis, Hepatopancreas, Hemolymph, Gill, Metabolism enzyme, Copper |
