| 摘要: |
| 采用稀碱浸提、蛋白酶水解与三氯乙酸沉淀相结合脱蛋白的工艺,对杂交鲟软骨提取琉酸软骨素的方法进行了较系统的研究。综合考察各种影响因素,设计了正交试验对提取工艺参数进行了优化,并对产品的质量指标进行了检验。结果表明,最佳提取工艺参数为:碱提取时料液比为1:2.0,碱浓度为4%,碱提温度为40℃;胰蛋白酶的酶解温度为55℃,酶量为2%,酶解时间为10h,制备的硫酸软骨素为白色粉末,得率为28.9%,纯度为92.3%,蛋白质含量为2.59%,各项质量指标完全符合标准要求。与目前已报道的其他软骨素制备工艺相比较,该方法具有工艺简便,提取率高,产品纯度高的特点。光谱分析法结构鉴定结果表明它是一种软骨素肽,其主要成分为硫酸软骨素C。 |
| 关键词: 杂交鲟，硫酸软骨素，提取工艺， 鉴定 |
| DOI：10.11693/hyhz200801008008 |
| 分类号: |
| 基金项目:国家863项目资助，2006AA100301号;浙江省科技支撑和引导计划项目，2007C33065号;浙江省高校青年教师资助项目，111041002号 |
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| EXTRACTION,ANALYSIS,AND IDENTIFICATION OF CHONDROITIN SULFATE IN STURGEON CARTILAGE |
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CHEN Xiao-E1, FANG Xu-Bo1, YU Hui1, SHI Jin-Yu1, WU Chang-Wen2
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1.College of Food and Pharmacy,Zhejiang Ocean University;2.Marine Science College,Zhejiang Ocean University
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| Abstract: |
| Preparation of chondroitin sulfate (Chs) from hybrid Sturgeon cartilage was introduced. The nuew procedures include dilute alkali extraction, and deprotein by the combination of enzyme hydrolysis and trichloroacetic acid precipitation. Upon the consideration of influential factors, technological parameters were optimized with orthogonal experiment for determining the physical and chemical properties of the Chs. The optimal conditions were 4% alkali extraction at 40℃ in material/solvent ratio at 1:2.0, while those of enzymatic hydrolysis were 55℃, 10h duration, and 2% of enzyme amount, under which white-powdered Chs was obtained at yield rate of 28.9%, purity of 92.3%, and protein content of 2.59%; the quality of the product well meets the quality standard. The procedures we used in this experiment are simpler with higher and purer yield than other ones previous reported. Having been identified in spectroscopy, the product is chondroitin sulfate-peptide, and excel Chs-C. |
| Key words: Hybrid Sturgeon cartilage, Chondroitin sulfate (Chs), Extraction technology, Identification |
