| 摘要: |
| 采用形态观察、培养特征、生理生化鉴定以及16S rDNA序列分析方法,对从宁波海域滩涂泥样中筛选到的一株放线菌XS904进行分类鉴定,同时对XS904菌株发酵液的抑菌活性和抑菌物质的理化性质进行了研究。结果表明,XS904菌株为链霉菌属灰浅红链霉菌(Streptomyces griseorubens)的变种;经液体培养,XS904菌株发酵液对革兰氏阳性细菌有显著的抑菌活性,对金黄色葡萄球菌的最小抑制浓度为0.78%;pH纸色谱和捷克八溶剂系统纸层析结果显示发酵液中的抑菌活性物质为一类中等极性的碱性抗生素,易溶于三氯甲烷,对温度较敏感,在酸性和中性条件下稳定。 |
| 关键词: 海洋放线菌 XS904 分类鉴定 发酵液 抑菌活性 |
| DOI：10.11693/hyhz200805006 |
| 分类号: |
| 基金项目:浙江省自然科学基金资助项目,402038号 |
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| IDENTIFICATION OF MARINE ACTINOMYCES XS904 AND THE ANTIMICROBIAL ACTIVITY OF ITS METABOLITE |
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YANG Wen-Ge1, LOU Qiao-Ming2, XU Da-Lun1, SUN Ai-Fei1, PAN Yun-Di3
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1.Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University, Ningbo, 315211;2.College of Food Science and Technology, Ocean University of China, Qingdao, 266003;3.Ningbo Entry-Exit Inspection and Quarantine Bureau, Ningbo, 315012
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| Abstract: |
| In searching for natural antibiotic resources, an actinomyces strain XS904 was discovered from marine mud ?ats in Ningbo, China. Based on the results of morphological, physiological-biochemical characteristics and 16S rDNA analysis, strain XS904 was identified as a variable species of Streptomyces griseorubens. Strain XS904 grew well on most tested media, producing abundant aerial and vegetative hyphae. However, the strain did not excrete dissoluble pigments. The spore chains of the strain XS904 were long-spiral, and the surface of spores was thorny and rugose. The vegetative hyphae had many branches without fragmentation. The metabolite of strain XS904 showed a strong inhibition against Gram-positive bacteria. The minimum inhibitory concentration of metabolite against Staphylococcus aureus was 0.78%. The results of Jack’s eight solvent system chromatography and pH paper chromatography of the fermentation extracts from strain XS904 showed that the main active components was a type of basic antibiotic. It has medium polarity, sensitive to temperature, and stable under acid and neural conditions but easily dissolved in chloroform. |
| Key words: Marine actinomyces, XS904, Classification and identification, Metabolite, Antimicrobial activity |
