| 摘要: |
| 用ATB微生物自动鉴定系统对分离自湖南湘潭地区人工养殖的患"红头病"的黄颡鱼体内的2株细菌(即HN004和HN005)进行了鉴定,发现它们的生理生化特征完全相同,均为革兰氏阴性短杆菌、接触酶阳性、吲哚阳性、H2S阳性;氧化酶阴性、V.P测定为阴性,与迟钝爱德华氏菌的表型特征非常相似。为进一步确定2株菌的分类学地位,测定了其16SrRNA基因序列,分析了相关细菌相应序列的同源性,构建分子系统发育树。结果表明,2菌株的序列完全一致,与迟钝爱德华氏菌的亲缘关系最近,相似性为99.0%。综合上述结果,2菌株可鉴定为迟钝爱德华氏菌(Edwardsiella tarda)。 |
| 关键词: 黄颡鱼 红头病 迟钝爱德华氏菌 分离 鉴定 |
| DOI：10.11693/hyhz200805013 |
| 分类号: |
| 基金项目:湖南省教育厅资助项目,B30512号;湖南科技大学博士基金资助项目,E50437号 |
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| ISOLATION AND IDENTIFICATION OF BACTERIOSIS PATHOGEN-EDWARDSIELLA TARDA FROM YELLOW CARTFISH (PELTEOBAGRUS FULVIDRACO) WITH RED HEAD DISEASE |
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DENG Xian-Yu1,2, LUO Wen2,3, TAN Shu-Hua1, QIU Shan-Hong4, CHEN Kang-Gui1
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1.School of Life Sciences, Hunan University of Science and Technology, Xiangtan, 411201;2.School of Zoological Sciences, Zhejiang University, Hangzhou, 310058;3.School of Life Sciences, Shaoxing University, Shaoxing, 312000;4.No.3 Middle School of Huaihua City of Hunan Province, Huaihua, 418000
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| Abstract: |
| Using ATB Microbiology Automatic Identification System, two bacterial strains HN004 and HN005 isolated from cultured yellow cat?sh (Pelteobagrus fulvidraco) infected with typical “Red head Disease” were identified. The two strains had completely the same physiological and biochemical characteristics: Gram-negative, rod-shaped, catalase-positive, indole-positive, H2S-positive, oxidase-negative, and V.P-negative; and were very similar to Edwardsiella tarda in most of the phenotypes. In order to determine the phylogenetic position of these two microorganism, their 16S rRNA genes were sequenced and compared with those of related strains. The molecular phylogenetic dendrogram was constructed based on the genetic distance analysis, which revealed that the 16S rDNA sequences of strain HN004 and HN005 were completely the same, and they exhibited the highest levels of similarity (99.0%) to E. tarda. Therefore, strains HN004 and HN005 were identified as E. tarda. |
| Key words: Pelteobagrus fulvidraco, Red head disease, Edwardsiella tarda, Isolation, Identification |
