| 摘要: |
| 设计了一套圆海链藻(Thalassiosira rotula)特异性探针, 运用双特异分子探针技术, 对圆海链藻(Thalassiosira rotula)进行了定性定量检测。结果表明,本实验中设计的一套探针与其它十几种藻无交叉反应, 具有种特异性; 细胞裂解液直接杂交检测优于提纯RNA 样品检测; 对自然样品做了初步检测, 发现天然海水中的其它浮游生物对该检测方法影响很小。
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| 关键词: 圆海链藻, 双特异分子探针技术, 赤潮藻检测 |
| DOI：10.11693/hyhz200901003003 |
| 分类号: |
| 基金项目:国家高技术研究发展计划(863 计划)资助项目, 2001AA635090 号; 国家高技术研究发展计划(863 计划)资助项目, 2003AA635010 号; 自然科学基金资助项目, 40406028 号。 |
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| DETECTION AND QUANTIFICATION OF THALASSIOSIRA ROTULA WITH DOUBLE SPECIFIC PROBE ASSAY |
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LI Xiu-Qin1, YU Zhi-Gang2, ZHEN Yu2, CAI Qing-Song3, MI Tie-Zhu4, LI Rong-Xiu5
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1.Marine Life Sciences College, Ocean University of China;2.Chemistry and Chemical Engineering College, Ocean University of China;3.Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences;4.Environmental Science and Engineering College, Ocean University of China;5.School of Life Science and Biotechnology, Shanghai Jiao Tong University
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| Abstract: |
| A double specific probe assay was applied to detect and quantify Thalassiosira rotula, a common species of harmful algae bloom, in which a set of oligonucleotide probes targeting 18S rRNA was used. The probes are species-specific, without obvious incident cross-reactivity with other species. Results of detection on the same number of cells to crude cell lysate and extracted total RNA were compared, showing that the former was better. A preliminary test was made on natural samples, and the noise produced by complex field background was minimal.
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| Key words: Thalassiosira rotula, Double specific probe assay, Harmful algae detection |
