| 摘要: |
| 在水温15.5—17.0℃的静水条件下, 以Hg2+、Cd2+和Se4+对刺参幼参进行了单一毒性和联合毒性实验。联合毒性实验采用等毒性配比法, 并以Marking 相加指数评价联合毒性效应。单一毒性实验结果表明, Hg2+、Cd2+、Se4+对刺参幼参急性毒性的96h 半致死质量浓度(LC50)分别为0.0912、4.6433 和0.7413mg/L, 三者对幼参的毒性大小依次为Hg2+> Se4+> Cd2+。Hg2+、Cd2+、Se4+对刺参幼参的最大容许质量浓度分别为0.0009、0.0464、0.0074 mg/L。联合毒性实验结果表明, 当Hg2+-Cd2+、Hg2+-Se4+以及Cd2+-Se4+分别以等毒性混合物共存时, 它们对刺参幼参在24h、48h、72h 和96h 的联合毒性均为拮抗作用; 当Hg2+-Cd2+-Se4+三者以等毒性混合共存时, 它们对刺参幼参在24h、48h、72h和96h 的联合毒性仍为拮抗作用。讨论了Hg2+、Cd2+、Se4+对刺参幼参联合毒性效应的机制。
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| 关键词: 刺参, Hg2+, Cd2+, Se4+, 急性毒性, 联合毒性 |
| DOI：10.11693/hyhz200902019019 |
| 分类号: |
| 基金项目:山东省科技攻关计划项目, 2008GG10005023 号; 鲁东大学科研基金重点项目, L20073305 号。 |
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| SINGLE AND JOINT TOXICITY OF MERCURY, CADMIUM AND SELENIUM ON JUVENILE SEA CUCUMBER APOSTICHOPUS JAPONICUS |
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SUN Zhen-Xing, WANG Hui-En, WANG Jing, LIU Jin-Chuan, ZHANG Jie, ZHANG Ying, WU Chuan-Yan
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College of Life Science, Ludong University
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| Abstract: |
| The single and joint toxicity of Hg2+, Cd2+ and Se4+ to the juveniles of the sea cucumber Apostichopus japonicus are tested in still water at water temperature of 15.5—17℃. The joint toxicity tests are determined using equitoxic ratio mixing, and the effects of joint toxicity are evaluated with the Marking’s additive index. The results indicate that the median lethal concentrations (LC50) of acute toxicity of Hg2+, Cd2+ and Se4+ at 96 hours to the juvenile are 0.0912, 4.6433 and 0.7413 mg/L, respectively. The toxicity sequence of the three poisons to the juveniles ranged as Hg2+ > Se4+ > Cd2+. The maximum permissible concentrations of Hg2+, Cd2+ and Se4+ on the juvenile are 0.0009, 0.0464 and 0.0074mg/L, respectively. The results of joint toxicity test show that when the juvenile are exposed to equitoxic mixtures, the joint toxicities of Hg2+ with Cd2+, Hg2+ with Se4+, and Cd2+ with Se4+ at 24, 48, 72 and 96 hours are all antagonistic. The joint toxicity are antagonistic too if Hg2+, Cd2+ and Se4+ are together with equitoxic mixture for 24, 48, 72 and 96 hours. Furthermore, the paper also discusses the possible mechanism of the joint toxicity acting on juvenile A. japonicus.
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| Key words: Sea cucumber Apostichopus japonicus, Hg2+, Cd2+, Se4+, Acute toxicity, Joint toxicity |
