| 摘要: |
| 从南极超微细菌ANT52(Alteromonas stellipolaris)提取粗外膜蛋白(Outer membrane protein, OMP)和粗脂多糖(Lipopolysaccharide, LPS), 分不同处理(OMP、LPS、OMP+LPS)免疫注射黑鲷, 在免疫后的第1、7、14、21、28、35 天时检测试验鱼的白细胞吞噬活性、溶菌酶活力、抗菌活力以及酚氧化酶活力等非特异性免疫指标的变化。结果表明, 自南极超微细菌ANT52 提取的LPS 及OMP均能够显著提高黑鲷的白细胞吞噬活性(P<0.05), 增强黑鲷血清中的抗菌活力、溶菌酶的活力和酚氧化酶活力(P<0.05)。各免疫处理对黑鲷免疫活性的影响效果不同, LPS、OMP 单独注射的免疫促进效果优于组合注射 (LPS+OMP); 且LPS、OMP 单独注射的黑鲷各免疫指标基本上在接种后的第28 天时最高, 而组合注射LPS+OMP 在接种后的第21 天最高。上述物质表现的免疫作用机理有待于进一步研究。
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| 关键词: ANT52 (Alteromonas stellipolaris), 外膜蛋白, 脂多糖, 黑鲷, 免疫活性 |
| DOI：10.11693/hyhz200902021021 |
| 分类号: |
| 基金项目:国家科技部国际合作重点资助项目, 2007DFA21300 号; 中德政府海洋科学双边合作项目, BMBF CHN 00/019 号; 宁波市科技局资助项目, 2004C100056 号 |
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| THE IMMUNITY OF ACANTHOPAGRUS SCHLEGELI INJECTED WITH OUTER MEMBRANE PROTEIN AND LIPPOLYSACCHRIDE OF ANTARCTIC ULTRAMICROBACTERIA ANT52 (ALTEROMONAS STELLIPOLARIS) |
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WANG Hai-Li1,2, YANG Ji-Fang1,2
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1.Municipal Key Laboratory of Microorganism and Environmental Engineering of Ningbo City;2.Faculty of Biological & Environmental Science of Zhejiang Wanli University
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| Abstract: |
| The immunity competence of Acanthopagrus schlegeli was studied by injecting with crude outer membrane protein (OMP) and lippolysacchride (LPS) extracted from Antarctic ultramicrobacteria ANT52 (Alteromonas stellipolaris) in different combinations. Four parameters: the phagocytic activity of leucocytes in blood, and the activities of lysozme, antibacteria, and phenoloxidase in serum of immunized fish, being used to represent the non-specific immunogenicity, were determined in 1, 7, 14, 21, 28, and 35 days after the injection. The results showed that the four parameters rose significantly during the experimental period (P<0.05). These parameters of single OMP or LPS treatment group were greater than those injected with OMP+LPS combination treatment group; and reached peak value in 28 days post vaccination. It indicates that immunization with OMP and LPS of ANT52 could stimulate and enhance the function of non-specific immunity of A. schlegeli. Future studies are needed to understand the mechanism of the immunity competence.
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| Key words: ANT52 (Alteromonas stellipolaris), Outer membrane protein, Lipopolysaccharide, Acanthopagrus schlegeli, Immunity competence |
