| 摘要: |
| 采用涂布法、划线分离法以及形态学和生理生化特性分析, 对湛江湖光岩微生物进行了分离纯化及部分性质研究; 同时用平板菌落计数法对其异养细菌、真菌和弧菌等的数量进行初步统计。采用牛津杯法, 对拮抗细菌进行筛选和培养特性研究。结果表明, 微生物数量与种类在水平分布上具有显著差异。底泥异养菌总量为(1.3—8.6)×108 cfu/g, 真菌总量为(0.1—5.7)×107 cfu/g。而水体细菌总量为(0.5—6.7)×105 cfu/ml, 真菌总量为(0.4—1.1)×103 cfu/ml。共分离纯化到148 株细菌, 经筛选发现16 株细菌对溶藻弧菌(Vibrio alginolyticus)具有明显的抑制作用, 占细菌总数的10.8%, 其中菌株DH078 和DH113 对溶藻弧菌的拮抗作用最明显。结果还表明, 细菌DH078 在营养肉汤中培养4d抑菌活性最强, 其产生抗菌活性物质的最佳条件是稀释2 倍的营养肉汤、蛋白胨0.6%、牛肉膏0.6%、酵母膏0.2%、盐度0 和pH 7.0。可见湖光岩微生物资源较为丰富, 本研究可为保护和充分开发利用
湖光岩微生物提供科学依据。
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| 关键词: 湖光岩(玛珥湖), 微生物, 拮抗菌, 培养条件, 筛选 |
| DOI：10.11693/hyhz200903020020 |
| 分类号: |
| 基金项目:广东省科技计划资助项目, 2006A20301003 号; 广东海洋大学自然科学基金资助项目, 0612050 号、0612190 号 |
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| THE ANTAGONISTIC BACTERIA FROM HUGUANGYAN MAAR LAKE |
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DING Yu1,2, JIAN Ji-Chang1,2, WU Zao-He1,2, BAI Li-Li1,2, LIU Jian-Feng1,2, LIN Dan1,2, YU Jun1,2
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1.Guangdong Provincial Key Lab of Pathogenic Biology and Epidemiology for Aquatic Economic Animals;2.Fisheries College, Guangdong Ocean University
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| Abstract: |
| Samples of bacterium taken from Huguangyan Maar Lake are isolated and purified using spread plate method and streak plate technique. The partial characteristics is analyzed morphologically and physiologically. The heterotrophic bacteria, fungi, and vibrio are assessed in plate colony-counting method. Antagonistic bacteria to vibrio are screened and their antagonistic ability studied using cup method. The results show a prominent difference in the quantity and species of microorganisms in different sites. The total numbers of heterotrophic bacteria and fungi are (1.3—8.6)×108 and (0.1—5.7)×107 cfu/g in sediment, while (0.5—6.7)×105 and (0.4—1.1)×103 cfu/ml in water, respectively. It was found that 16 bacteria strains, which account for 10.8% of the total (148), are obviously inhibitive to Vibrio alginolyticus, and bacteria DH078 and DH113 have a significant inhibitive zone. The results also show that antibiotic activity of DH078 is the strongest after being cultured for four days in nutrient broth medium. The optimal cultural conditions for their antibiotic ability are: broth medium of 2-time dilution, 0.6% peptone, 0.2% yeast extract, 0.6% beef extract, 0 salinity, and pH 7.0. It is obvious that the microorganism resources in Huguangyan Maar Lake are quite abundant and valuable. The scientific foundation for protection and sufficient utilization of these microorganism resources are also provided in this paper.
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| Key words: Huguangyan Maar Lake, Microorganism, Antagonistic bacterium, Cultural condition, Screening |
