| 摘要: |
| 利用15 对微卫星标记对人工繁育的岱衢族(DQ1 和DQ2)、闽-粤东族(MY1 和MY2)及正交(DM1 和DM2)和反交(MD 和MD21) F2 及F3 连续两代共8 个群体的250 个样品进行了遗传多样性及遗传差异检测。15 个位点在所有检测群体中均为高度多态, 共检测等位基因215 个, 每个位点的平均等位基因数是8.1—11.7, 平均观察和期望杂合度分别是0.598—0.790 和0.732—0.794。F2 和F3代内的等位基因频率无明显差异(P>0.05), 而两代间在15 个位点的等位基因频率上存在约34.13%的遗传差异; Fst 值检测发现, F2 的4 个群体间的Fst 值是0.028—0.067, 平均0.0429, F3 的Fst 值是0.037—0.068, 平均0.0535。经过一代繁殖, F2 和F3 之间的遗传分歧平均增加了1% (0.0106); 群体间的N-J聚类图显示, 8 个群体虽然聚成一族, 但是F2 和F3 之间的遗传距离有增大的趋势。以上结果表明, 随着人工选育力度的加强, 岱衢族和闽-粤东族大黄鱼群体间的遗传距离由近及远, 遗传分歧有加大的趋势。
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| 关键词: 大黄鱼, 微卫星标记, 遗传多样性, 遗传分化 |
| DOI：10.11693/hyhz200904005005 |
| 分类号: |
| 基金项目:国家“863”计划资助项目, 大黄鱼优质抗逆新品种培育(2006AA10A405 号) |
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| GENETIC DIFFERENTIATION BETWEEN F2 AND F3 CULTURED POPULATIONS IN LARGE YELLOW CROAKER PSEUDOSCIAENA CROCEA |
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CHANG Yu-Mei1, WANG Wen-Wen2, XU Wan-Tu3, LI Ming-Yun4, XUE Liang-Yi4, LIANG Li-Qun1
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1.Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences;2.The College of Life Sciences, Nankai University;3.Xiangshan Seaport Aquatic Seedling & Fingerling Limited Company;4.Life Science and Biotechnology Faculty, Ningbo University
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| Abstract: |
| Large yellow croaker, Pseudosciaena crocea, is one of the economically important marine fish in China. Four populations bred consecutively for two generations (F2 and F3) were studied with genetic variability using 15 microsatellite markers. A total of 250 individuals comprised population Daiqu (DQ1 and DQ2), population Min-Yue (MY1 and MY2) and their reciprocal crosses (DM1, DM2, MD1 and MD2). The genetic variability of 15 loci was high for all the populations. The mean numbers of alleles per locus ranged from 8.1—11.7, while the mean observed and expected heterozygosity ranged from 0.598 to 0.790, and from 0.732 to 0.794, respectively. There was no significant difference in allele frequencies within F2 and F3 (P>0.05); however, approximately 34.13% differences were found between them. In addition, the average values of Fst between F2 and F3 were 0.0429 and 0.0535 respectively, having increased by 1% of genetic divergence per generation. The Neighbor-Joining tree topology constructed on the basis of genetic distances among eight populations showed also such a tendency, i.e. the genetic distance was generally increased between F2 and F3 during breeding. The results mentioned above demonstrate that artificially selective breeding would strengthen the genetic divergence between F2 and F3, especially populations Daiqu and Min-Yue.
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| Key words: Large yellow croaker, Microsatellite, Genetic variability, Genetic differentiation |
