| 摘要: |
| 采用AFLP 技术方法, 对福建二个野生及二个养殖大黄鱼群体进行了遗传多样性的研究。实验采用7 对引物组合, 在四个群体的120 个个体中共扩增出472 个位点, 其中多态位点为220 个,多态位点比例为46.61%。结果表明, 大黄鱼养殖群体的遗传多样性降低：宁德野生群体和湄州野生群体多态位点比例分别为42.42%和45.14%, Nei 遗传多样性指数分别为0.1046 和0.1245, Shannon 多样性指数分别为0.1659 和0.1942, 平均有效等位基因数(Ne)分别为1.4153 和1.4428; 宁德养殖群体和连江养殖群体多态位点比例分别为40.83%和40.53%, Nei 遗传多样性指数分别为0.1027 和0.1039,Shannon 多样性指数分别为0.1615 和0.1633, 平均有效等位基因数(Ne)分别为1.3919 和1.3856。四个群体间遗传距离为 0.0359—0.1465, 平均为0.079, 群体间基因流为1.0808。
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| 关键词: 大黄鱼, AFLP, 养殖群体, 遗传多样性的降低 |
| DOI：10.11693/hyhz200904009009 |
| 分类号: |
| 基金项目:福建省高等教育新世纪优秀人才支持计划项目, 闽教科[2006]35 号; 集美大学创新团队基金项目, 2007A001 号 |
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| LOSS OF THE GENETIC DIVERSITY IN CULTIVATED POPULATIONS OF PSEUDOSCIAENA CROCEA BY AFLP |
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LI Zhong-Bao1,2, FANG Xiu3, CHEN Jin1,2, CHANG Jian-Bo1,2, LEI Guang-Gao1,2, ZHANG Gui-Ling1,2, ZHAO Bin-Li1,2, WANG Zhan-Lin1,2
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1.Fisheries College, Jimei University;2.Institute of Aquaculture Biotechnology, Jimei University;3.Fujian Min Wei Aquatic Products Industrial Co., Ltd.
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| Abstract: |
| Genetic diversity of two wild and two cultivated populations of Pseudosciaena crocea in Fujian Province were analyzed by AFLP. A total of 472 loci were detected from 120 individuals in the 4 populations, of which 220 (46.61%) are polymorphic. The results from AFLP technique show that the genetic diversity in the cultivated populations has been reduced compared to that of wild populations. The percentage of polymorphic loci, the Nei’s Gene Diversity, Shannon’s Information Index and the mean effective number of alleles per locus in two wild populations are 42.42%—45.14%, 0.1046—0.1245, 0.1659—0.1942 and 1.4153—1.4428, respectively, which in two cultivated populations are 40.83%—40.53%, 0.1027—0.1039, 0.1615—0.1633 and 1.3919—1.3856, respectively. Genetic distance among the 4 populations varies from 0.0359—0.1465, in the mean of 0.079, and the gene flow (Nm) is 1.0808. These results show a lower-level genetic diversity compared to other fishes.
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| Key words: Pseudosciaena crocea, AFLP, Cultivated population, Loss of genetic diversity |
