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| 黄颡鱼(Pelteobagrus eupogon)不同生态地理分布群体遗传多样性的微卫星分析 |
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李大宇1,2, 殷倩茜1,3, 侯 宁1,3, 孙效文1, 梁利群1
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1.中国水产科学研究院黑龙江水产研究所 农业部北方鱼类生物工程育种重点开放实验室;2.中国水产科学研究院淡水渔业研究中心 农业部水生动物遗传育种和养殖生物学重点开放实验室;3.大连水产学院生命科学与技术学院
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| 摘要: |
| 利用40 个黄颡鱼微卫星分子标记对吉林省月亮湖野生黄颡鱼(YL, 30 尾)、四川野生黄颡鱼(SC, 30 尾)、黑龙江省哈尔滨市松花江段黄颡鱼(SH, 18 尾)、湖北省荆州市长湖野生黄颡鱼子代(YY, 30 尾)及其亲本(QQ, 30 尾)、天津市换新国家级原良种场黄颡鱼1 号(TJ, 30 尾) 6 个黄颡鱼群体的观
测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)和有效等位基因数(Ae)等进行了遗传检测, 根据基因频率计算遗传相似系数和Nei 氏标准遗传距离, x2 检验估计Hardy-Weinberg 平衡, 采用近交系数(FST)和基因流(Nm)分析群体的遗传分化及其来源。同时, 使用PHYLIP3.63 软件绘制基于Nei 氏标准遗传距离的UPGMA 进化树。6 个群体共检测到5042 个扩增片段, 长度在150—650bp, 40 个基因座扩增出等位基因数从1—8 个不等, 共计143 个等位基因, 平均每个基因座扩增得到3.575 个等位基因。结果显示：(1) 6 个黄颡鱼群体的多态性指标均适中, PIC 在0.00—0.79 之间, 平均值为0.40、0.37、0.35、0.39、0.35 和0.19, 有效等位基因数(Ae) 1.00—5.44 个不等, 平均有效等位基因数依次为2.05、2.30、1.93、2.07、1.93 和1.47, 无偏期望杂合度(He)在0.00—0.83 之间, 平均值为0.45、0.46、0.36、0.46、0.41 和0.23; (2) 遗传相似系数YY 与QQ 遗传相似度最高(0.9947), TJ 与SC 遗传相似系数最低(0.4846), 聚类结果与地理分布呈一定相关性。
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| 关键词: 黄颡鱼, 遗传多样性, 分子标记, 微卫星 |
| DOI：10.11693/hyhz200904011011 |
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| 基金项目:国家重点基础研究发展计划, 2004CB117405 号 |
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| GENETIC DIVERSITY OF DIFFERENT ECOLOGO-GEOGRAPHICAL POPULATIONS OF YELLOW CATFISH PELTEOBAGRUS EUPOGON |
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LI Da-Yu1,2, YIN Qian-Qian1,3, HOU Ning1,3, SUN Xiao-Wen1, LIANG Li-Qun1
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1.Key Open Laboratory for Bioengineering Breeding of Northern Fishing, Ministry of Aquaculture, Heilongjiang Fisheries Research Institute, Chinese Academy of Fishery Sciences;2.Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biolo;3.College of Aqua-life Science and Technology, Dalian Fisheries University
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| Abstract: |
| Forty microsatellite loci were used for analyzing six populations of yellow catfish Pelteobagrus eupogon: YL [the Yueliang (Moon) Lake, Jilin Province, 30 samples], SC (Sichuan Province, 30 samples), SH (Songhua River at Harbin, Heilongjiang Province, 18 samples), YY (the Changhu Lake, Jingzhou, Hubei Province, 30 samples), QQ (the parent generation of YY, from the Changhu Lake also), and TJ (Huanxin Breeding Farm, Tianjin, 30 samples). The observed (Ho), expected (He) heterozygosity, polymorphic information content (PIC), and number of effective alleles (Ae) were determined. Genetic similarity index and genetic distance were computed on the allele frequency. The Hardy-Weinberg Equilibrium was checked by x2 Test. Genetic differentiation (FST) and hierarchical partition of genetic diversity (Nm) were used. A dendrogram was built on the UPGMA results using PHYLIP software package (Ver.3.63). A total of 5042 fragments ranging from 150bp and 650bp in length were obtained. One to eight alleles were amplified in 40 loci and 143 alleles in all six populations. The average number of alleles in each locus is 3.575. The result shows that 1) the level of genetic variability is moderate in the six populations. The polymorphic information contents of the six populations are from 0.00 to 0.79, in average at 0.40, 0.37, 0.35, 0.39, 0.35 and 0.19, respectively. Effective alleles are from 1.00 to 5.44, in average of 2.05, 2.30, 1.93, 2.07, 1.93 and 1.47, respectively. The expected heterozygosity ranges from 0.00 to 0.83, in average at 0.45, 0.46, 0.36, 0.46, 0.41 and 0.23, respectively; 2) the highest genetic similarity index at 0.9947 was found between YY and QQ; and the lowest index at 0.4846 between SC and TJ. Correlation between the clustering result and the geographical distribution may exist.
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| Key words: Yellow catfish Pelteobagrus eupogon, Genetic diversity, Molecular marker, Microsatellite |
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