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| 黑鲪(Sebastes schtegeli)遗传多样性的等位酶研究 |
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刘丽娟1, 任利华2, 姜向阳2, 宋秀凯2, 刘爱英2, 杜荣斌3, 张 静4, 姜海滨2
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1.山东省海洋水产研究所, 山东师范大学生命科学学院;2.山东省海洋水产研究所;3.烟台大学海洋学院;4.山东省海洋水产研究所,上海海洋大学水产与生命学院
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| 摘要: |
| 应用垂直板聚丙烯酰胺凝胶电泳(PAGE)技术, 对分别取自青岛、日照、荣成、长岛的野生黑鲪及荣成黑鲪人工繁育的F1 代共5 个群体的等位酶多态性进行分析。结果表明, 分析的5 个群体13 种等位酶的25 个基因座位中, 多态位点比例(P)为36.0%—20.8%, 平均每个位点的预期杂合度(He)为0.076—0.036, 平均每个位点的实际杂合度(Ho)为0.063—0.040, 证明所选5 个群体具有较高的遗传多态性。5 个群体之间遗传距离(D)为0.024—0.001, 根据遗传距离进行UPGMA 聚类分析, 5 个群体聚类次序与亲缘关系和地理位置表现出明显的正相关关系, 长岛群体与其它四个群体间遗传距离最大(0.024—0.020), 达到居群间差异水平。
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| 关键词: 等位酶, 黑鲪, 遗传多样性 |
| DOI：10.11693/hyhz200904013013 |
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| 基金项目:山东省科技厅良种工程项目“优质抗病速生鱼类(黑鲪)良种选育”课题资助, 2005—2008。 |
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| ALLOZYME AND GENETIC DIVERSITY OF BLACK ROCKFISH SEBASTES SCHTEGELI |
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LIU Li-Juan1, REN Li-Hua2, JIANG Xiang-Yang2, SONG Xiu-Kai2, LIU Ai-Ying2, DU Rong-Bin3, ZHANG Jing4, JIANG Hai-Bin2
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1.Shandong Marine Fisheries Research Institute,School of Life Science, Shandong Normal University;2.Shandong Marine Fisheries Research Institute;3.School of Oceanology, Yantai University;4.Shandong Marine Fisheries Research Institute,School of Fishery and Life Science, Shanghai Ocean University
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| Abstract: |
| Allozymes of five populations of black rockfish Sebastes schtegeli collected from the coastal areas of Shandong Peninsula are isolated and analyzed with polyacrylamid gel electrophoresis (PAGE). 25 loci in 13 enzymes are examined. Result shows that the mean proportion of polymorphic loci (P) ranges 36.0%—20.8%, predicted heterozygosity (He) 0.076—0.036, and actual heterozygosity (Ho) 0.063—0.040, showing a large genetic diversity among the 5 populations with the genetic distance (D) from 0.024 to 0.001. The result of UPGMA shows that the cluster reflects a very positive correlation among populations and locations. In terms of genetic distance, Changdao population is far from other 4 populations, and reaches the level of different geographical population.
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| Key words: Allozyme, Black Rockfish Sebastes schtegeli, Genetic diversity |