| 摘要: |
| 采用单养和混养的方法, 在实验条件下研究了东海原甲藻对中华哲水蚤摄食和消化酶活性的影响。结果表明：(1) 中华哲水蚤对东海原甲藻存在一定摄食行为, 藻类密度对摄食率有明显的影响。实验密度下, 中华哲水蚤对东海原甲藻的最大摄食率为930cells/(ind·h)。滤水率随着藻密度的增加而呈单一性的下降; (2) 混养条件下, 中华哲水蚤对金藻和东海原甲藻的摄食率均较单养时下降,滤水率的变化与摄食率相似; (3) 不同藻密度下, 昆布多糖酶活性都明显高于麦芽糖酶和纤维二糖酶的活性, 而麦芽糖酶活性又稍高于纤维二糖酶的活性。与金藻相比, 东海原甲藻实验组中华哲水蚤3种消化酶活性明显升高(P<0.05)。
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| 关键词: 东海原甲藻, 中华哲水蚤, 摄食, 消化酶活性 |
| DOI：10.11693/hyhz200904016016 |
| 分类号: |
| 基金项目:新世纪优秀人才计划项目, NCET-05-0597 号; 浙江省高校优秀青年教师资助计划, 2008.12—2009.12; 宁波市自然科学基金项目, 2008A610075 号; 宁波大学人才和学科项目, RCL2008002 、XK0715048 号 |
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| EFFECT OF PROROCENTRUM DONGHAIENSE ON FEEDING AND DIGESTIVE ENZYME ACTIVITY OF CALANUS SINICUS |
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XIE Zhi-Hao1,2, WANG You2, TANG Xue-Xi2
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1.Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University;2.Marine Ecology Laboratory, Ocean University of China
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| Abstract: |
| Effect of Prorocentrum donghaiense on feeding and digestive enzyme activity of marine planktonic copepod Calanus sinicus, a key species of Yellow Sea and East China Sea, was studied using single and mixed culture methods under controlled laboratory conditions. The results show that: (1) C. sinicus takes some P. donghaiense with the maximum ingestion rate (IR) of 930 cells/(ind·h), and the IR is closely related with the microalga density. Differently, the clearance rate (CR) decreases monotonically with microalga density increasing during the whole experiment. (2) The IRs of C. sinicus on Isochrysis galbana and P. donghaiense in mixed cultures are lower than those of two microalga species in single culture. (3) The digestive enzyme activity of C. sinicus changes with microalga species and density. The activity of laminarinase is higher than those of maltase and cellobiase, and cellobiase has little lower activity than those of other two. The result also shows that the microalga species affects the digestive enzyme activity. C. sinicus fed with P. donghaiense showed relatively higher digestive enzyme activity than those fed with I. galbana (P<0.05).
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| Key words: Prorocentrum donghaiense, Calanus sinicus, Feeding, Digestive enzyme activity |
