| 摘要: |
| 从湘潭南天化工厂的甲胺磷废水和污泥中分离细菌样品, 以甲胺磷为唯一碳源和能源, 经过定向筛选, 得到1 株可高效降解甲胺磷的菌株HN003, 气相色谱测定其对甲胺磷的平均降解效率在24h、48h 和72h 分别达到45.8%、88.5%和100%。对其进行常规生理生化测试, 结果表明, 菌株HN003 革兰氏染色为阳性, 在10—42℃、pH 3.0—pH 14.0 范围内均能生长, 并与巴氏葡萄球菌的表型特征非常相似。为了进一步确定HN003 的分类学地位, 测定了其16S rRNA 基因序列(1541
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| 关键词: 甲胺磷, 巴氏葡萄球菌, 降解, 生理生化测试, 16S rRNA 基因 |
| DOI：10.11693/hyhz200905005005 |
| 分类号: |
| 基金项目:国家“863”计划资助项目，2005AA219040 号; 湖南科技大学博士基金资助项目, E50437 号 |
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| STAPHYLOCOCCUS PASTEURI, AN EFFICIENT BIODEGRADER TO METHAMIDOPHOS: ISOLATION AND MOLECULAR IDENTIFICATION |
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DENG Xian-Yu1, GAO Jian2, TAN Shu-Hua2, XIANG Yan-Ci2
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1.School of Life Sciences, Hunan University of Science and Technology,Cycle Eco-Agriculture Company Ltd.;2.School of Life Sciences, Hunan University of Science and Technology
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| Abstract: |
| Using methamidophos as the sole carbon and energy source, a bacteria strain HN003 was isolated and purified from methamidophos-polluted sludge from a local chemical factory discharge in Xiangtan, Hunan Province. The strain showed an efficient biodegrading ability to methamidophos. The methamidophos-degrading rate of this strain reached 45.8%, 88.5%, and 100% in 24 h, 48h, and 72h, respectively, determined in gas-chromatography. Results of traditional physiological and biochemical tests show that the strain is Gram-positive, and can grow in 10—42℃, and pH 3.0—14.0. The strain is very similar to Staphylococcus pasteuri in most of the phenotypes in traditional physiological and biochemical aspects, as well as in molecular phylogenetic dendrogram built from 1541bp 16S rRNA gene sequence with which they are compared. Therefore, strain HN003 was identified as S. pasteuri.
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| Key words: Methamidophos, Staphylococcus pasteuri, Degradation, Physiological and biochemical tests, 16S rRNA gene |
