| 摘要: |
| 采用培养与16S rRNA 基因序列比对的方法, 对海水网箱养殖区底泥中的可培养细菌进行研究。结果表明, 网箱正下方的产芽孢细菌比例高达50%, 随着离网箱距离的增加, 产芽孢细菌的比例明显降低, 200m 远时, 其比例降至17%。从3 个采样点分离纯化出91 株产芽孢细菌, 对其菌落形态、菌体形态的研究和部分菌株16S rRNA 基因的DGGE 带型和序列分析表明, 91 株产芽孢细菌中,有85 株属于芽孢杆菌属(Bacillus)的10 个种, 25 个发育型; 6 株属于盐芽孢杆菌属
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| 关键词: 产芽孢细菌, 海水网箱养殖, 底泥, 16S rRNA 基因, 多样性 |
| DOI：10.11693/hyhz200905015015 |
| 分类号: |
| 基金项目:浙江省自然科学基金人才培养项目, R305333 号; 长江学者和创新团队发展计划(PCSIRT)资助, “高效海水养殖与灾害响应机制”, IRT0734 号; 国家科技基础条件平台建设项目(2005DKA2120609)资助 |
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| DIVERSITY OF SPORE-FORMING BACTERIA IN MARINE SEDIMENTS BELOW FISH-CAGE |
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WEN Dan, ZHANG De-Min, CHU Hang
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Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Faculty of Life Science and Biotechnology, Ningbo University
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| Abstract: |
| Spore-forming bacteria have been widely studied for their capability to secrete various extracellular enzymes such as proteaes, amylase etc., but less for their distribution and ecological roles in natural environment. The diversity of spore-forming bacteria in marine sediments of cage culture area were investigated in this paper. Results indicate that the proportion of spore-forming bacteria in sediment just below cage is as much as 50% of culturable heterotrophic bacteria. Distant from cage, the proportion of spore-forming bacteria reduces significantly. At a place 200m distant, the proportion dropped to 17%. Ninety one strains of spore-forming bacteria in three localities were isolated. Based on the sequences of 16S rRNA gene, these bacteria can be grouped into 2 genera. Of the 91 strains, 85 belong to Bacillus, including 10 species, 25 phylotypes; six strains are from 1 species of Halobacillus, containing 3 phylotypes. B. marisflavi, B. baekryungensis, B. aquaemaris and B. pumilu are dominant groups. As ecological function, about half of the spore-forming bacteria can metabolize sulfide, some can reduce nitrate or break down phosphate compound, and only 3 strains can reduce nitrite.
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| Key words: Spore-forming bacteria, Marine cage culture, Sediment, 16s rRNA gene, Diversity |
