| 摘要: |
| 以实验室单种培养的强壮前沟藻为对象, 研究了强壮前沟藻毒素的来源, 分析了藻毒素浓度与溶血活性的相关性, 研究了理化因子对藻毒素溶血活性的影响。结果表明, 溶血活性随着强壮前沟藻细胞的增殖呈上升的趋势, 细胞内容物和细胞碎片的溶血活性显著高于去藻过滤液, 强壮前沟藻毒素主要来源于衰亡期的细胞内容物和细胞碎片, 并存在浓度相关的溶血性。强壮前沟藻毒素的溶血活性不存在光敏感性。随着pH 的上升, 毒素溶血活性呈降低趋势。Ca2+、Mn2+、Cu2+抑制了强壮前沟藻毒素溶血活性, 而Co2+、Zn2+显著的增强了溶血活性, Mg2+对其溶血活性的影响不显著。EDTA 对强壮前沟藻毒素的溶血活性有显著的促进作用。
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| 关键词: 强壮前沟藻, 毒素, 溶血活性 |
| DOI：10.11693/hyhz200906010010 |
| 分类号: |
| 基金项目:山东省自然科学基金项目, Q2007D06 号; 青岛农业大学高层次人才启动基金项目, 630642 号 |
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| HEMOLYTIC ACTIVITY OF TOXIN IN AMPHIDINIUM CARTERAE HULBURT |
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GE Wei1, WANG Jin-Ye2, CHAI Chao3,4
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1.College of Life Science, Qingdao Agricultural University;2.College of Animal Science, Qingdao Agricultural University;3.Resource and Environmental College, Qingdao Agricultural University;4.Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences
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| Abstract: |
| Aggravating eutrophication in coastal zones resulted in larger and more frequent harmful algae with more toxic substances to local habitats. Toxic products of the secondary metabolism, such as dinoflagellates, are become a focus of research. Among them, Amphidinium carterae Hulburt is thought to be one of the toxin-producing species. For the first time in China, hemolytic toxin from A. carterae is carried out in this research. The dinoflagellate was cultured separately in laboratory; the source of toxin the relationship of toxin with hemolytic activity, and the effect of physical and chemical factors on the hemolytic activity were studied. The results show that the hemolytic activity increases with the proliferation of A. carterae. The hemolytic degree is low in exponential phase, intermediate in stationary phase, and high in decline phase (>70%). The hemolytic activity in cellular content and cell debris is significantly higher than that in the algae-free supernatant. Therefore, the toxin of A. carterae is mainly from cellular content and cell debris. Correlation study between the toxin and hemolytic activity shows that with increasing dilution gradient, the hemolytic activity decreases obviously. In the gradient at 1/16, the hemolytic degree is close to that of the control, and irrelevant to lighting as it reached almost 90% in both dark and light conditions. Therefore, no photosensitizing hemolytic activity existed. The hemolytic activity decreases with pH value: high at pH 5—6, and very low at pH 7.2—8. The result also shows that metal ions Ca2+, Mn2+ and Cu2+ inhibit the hemolytic activity by 5.8%—60%, while Co2+ and Zn2+ enhance it by 7.0% and 10.8% respectively, and Mg2+ shows no significant effect. In addition, EDTA can enhance the hemolytic activity significantly as an intercalating agent of metal.
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| Key words: Amphidinium carterae Hulburt, Toxin, Hemolytic activity |
