| 摘要: |
| 采用超滤法、聚丙烯酰胺凝胶电泳法、CM-Sepharose FF 阳离子交换柱层析法等对牡蛎软体中抗菌蛋白(Antibacterial protein, AP)进行分离纯化, 并采用体外实验观察其对常见致病菌的抗菌作用。结果表明, 此蛋白分子量在16.4—39 kDa, 蛋白质含量≥70%。抗菌蛋白对生孢梭菌、白色念珠菌、变形杆菌和金黄色葡萄球菌、表皮葡萄球菌、大肠埃希氏菌、铜绿假单胞菌、肺炎克雷伯杆菌、肠球菌、不动菌属均有较强的抗菌作用, MIC 范围在0.125—32.0 μg/ml 之间, MBC 范围在0.250—1.00 mg/ml 之间。以透射电镜观察AP 对大肠杆菌的作用, 提示 AP 可能通过作用于菌体细胞壁,发挥其抗菌作用。
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| 关键词: 牡蛎软体, 蛋白, 制备, 抗菌作用 |
| DOI：10.11693/hyhz201001004004 |
| 分类号: |
| 基金项目:国家863 计划资助项目, 2003AA625070 号; 山东省科技厅资助项目, 22130107 号 |
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| PREPARATION AND ANTIBACTERIAL ACTIVITY OF OYSTER PROTEIN |
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WANG Chun-Bo1, LAN Xiao-Ming1,2, WANG Hong-Jiang1, MU Qian1,2, SUN Mi3
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1.Qingdao University Medical College;2.Qingdao Health School;3.Yellow Sea Fisheries Research Institude Chinese Academy of Fishery Sciences
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| Abstract: |
| Antibacterial protein (AP) was prepared and purified by means of ultrafiltration technology and CMSepharoe FF positive ion column chromatography from oyster muscle. The in vitro activity of AP was also studied. The AP demonstrated in vitro activity against test strains, including Clostridium sporogenes, Candida albicans, Bacillus proteus, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterococcus, and Acinetobacter. The MIC range and the MBC range of the AP against these stains was 0.125—32.0μg/mL and 0.250—1.00 mg/ml, respectively. In addition, after treated with AP, the cell walls of pathogens showed obvious morphologic changes, showing that the effective places of the AP on bacteria were at the cell walls.
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| Key words: Muscle of oyster, Protein, Preparation, Antibacterial activity |
