| 摘要: |
| 采用二因素三水平设计试验, 用添加不同水平配比的VE 和Se(mg/kg)的饵料G0(0, 0)、G1(200, 0.2)、G2(200, 0.4)、G3(200, 0.8)、G4(400, 0.2)、G5(400, 0.4)、G6(400, 0.8)、G7(800, 0.2)、G8(800,0.4)、G9(800, 0.8)投喂凡纳滨对虾, 研究VE 和Se 对体长3cm 左右凡纳滨对虾体液抗氧化系统的影响, 试验进行4 周。结果表明, 第2 周时, VE、VE 和Se 对O2?、酚氧化酶(PO)、血清总抗氧化能力(T-AOC)有显著影响(P<0.05); Se 对PO、血清和肝胰腺中T-AOC 以及肝胰腺中GPx 有显著影响(P<0.05)。第4 周时, VE 和Se 协同作用对4 种抗氧化指标都有显著影响(P<0.05)。第2、4 周时, 抗氧化酶活力比第0 周显著升高。对O2?的研究发现, 在第2 和第4 周时, G8 组血细胞O2?最多。第2周时, 血清PO 在G5、G9 组活力最高(P<0.05), 两组间差异不显著(P>0.05); 血清和肝胰腺中T-AOC在G2、G3 组最高(P<0.05); 对虾肝胰腺中GPx 酶活力在G3 和G5 组达到最高(P<0.05), 两组间差异不显著(P>0.05)。第4 周时, G7 组PO 和GPx 活力最高, G9 组T-AOC 活力达到最高(P<0.05)。研究结果提示: 添加适量的VE 和Se 能显著提高对虾抗氧化能力, VE 和Se 对抗氧化系统具有随时间变化的动态调节作用, VE 和Se 之间存在交互作用。当在基础饵料中分别添加VE和Se 在400mg/kg、0.4mg/kg时, 凡纳滨对虾机体抗氧化能力整体达到平衡, 能有效抵制氧自由基的损伤。
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| 关键词: 凡纳滨对虾, 硒, 维生素E, 抗氧化酶 |
| DOI：10.11693/hyhz201001009009 |
| 分类号: |
| 基金项目:广东省自然科学基金重点项目, 021098 号、5005909 号; 广东省科技计划项目, 2002C20304 号、2007B020708013 号; 广州市科技攻关项目, 2002Z2-E0102 号; 广东省教育部产学研结合专项资金项目, 2006D90204005 号; 国家自然科学基金资助项目,30671628 号; 国家科技支撑计划课题, 2007BAD29B04 号、2007BAD29B06 号。 |
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| EFFECTS OF DIETARY VITAMIN E AND SELENIUM ON THE ANTIOXIDANT SYSTEM OF SHRIMP LITOPENAEUS VANNAMEI |
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HU Jun-Ru1,2, WANG An-Li1, CAO Jun-Ming3
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1.Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, College of Life Science, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University;2.Institute of Animal Sc;3.Institute of Animal Science, Guangdong Academy of Agricultural Sciences
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| Abstract: |
| Litopendeus vannamei (PL 3cm) samples were divided randomly into 10 groups in triplication, each contained 30 individuals. A matrix of three-level by two-factor was designed. The shrimp were fed with vitamin E and selenium for each group, respectively as: G0 (0, 0), G1 (200, 0.2), G2 (200, 0.4), G3 (200, 0.8), G4 (400, 0.2), G5 (400, 0.4), G6 (400, 0.8), G7 (800, 0.2), G8 (800, 0.4), and G9 (800, 0.8mg/kg). The experiment lasted for four weeks during which the effects of different levels of vitamin E and selenium on O2? in haemocytes, Phenoloxidase (PO) activity and total-antioxidative activity (T-AOC) in serum and hepatopancreas, and glutathione peroxidase (GPx) activity in hepatopancreas, were studied. The antioxidant activity was checked at 0, 2, and 4 weeks. The result shows that vitamin E and selenium could enhance the antioxidant ability dynamically. The optimal point of adding scheme was at 400mg/kg vitamin E and 0.4mg/kg selenium into the basal diets, at which the overall antioxidant system of the shrimp would reach the balance for fighting against the damage by oxygen radical.
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| Key words: Litopendeus vannamei, Selenium, Vitamin E, Antioxidant enzyme |
