| 摘要: |
| 针对饥饿对大菱鲆雌核发育二倍体仔鱼前期生长发育的影响进行研究, 并对仔鱼的饥饿不可逆点进行了测定。结果表明, 水温14.5—16℃的培育条件下, 大菱鲆雌核发育饥饿仔鱼卵黄于5—6日龄消耗殆尽, 油球6—7 日龄消耗殆尽; 饥饿仔鱼最高初次摄食率出现在4 日龄, 为85.3%; 仔鱼的饥饿不可逆点出现在8 日龄; 饥饿仔鱼全长生长速度显著慢于投喂仔鱼, 6 日龄后全长呈负增长, 并且出现胸角突出、体色暗黑、消化道萎缩等较为明显的饥饿特征。本实验同时对大菱鲆常规二倍体仔鱼的饥饿耐受情况对照研究, 表明, 常规二倍体饥饿仔鱼的卵黄和油球消耗规律、初次摄食率变化、PNR 点及全长生长与雌核发育仔鱼无显著差异。
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| 关键词: 大菱鲆, 雌核发育二倍体仔鱼, 饥饿, 不可逆点 |
| DOI：10.11693/hyhz201002006006 |
| 分类号: |
| 基金项目:农业部公益性行业科研重大专项“鲆鲽类全雌苗种大规模培育技术研究”, nyhyzx07-046 号; 国家鲆鲽类产业技术体系建设专项资金, nycytx-50-G2 号; 国家自然科学基金青年项目“下丘脑神经肽(Orexin)及其受体调控大菱鲆仔鱼内-外源营养转换的机制研究”, 30901111 号。 |
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| EFFECT OF STARVATION ON DEVELOPMENT OF EARLY-STAGE LARVAE OF GYNOGENESIS TURBOT (SCOPHTHALMUS MAXIMUS L.) |
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DING Fu-Hong1, LEI Ji-Lin1, LIU Xin-Fu1, MENG Zhen1, LIU Bin1, ZHANG He-Sen2, MA Ai-Jun1
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1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology;2.Qingdao General Aquaculture Co. Ltd.
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| Abstract: |
| Starvation is a major reason causing mortality during early feeding stage of fish larvae. We conducted an experiment to study how the larvae endure starvation via absorption, transition, and disappearance of inner nutrition materials, yolk, and oil globule. The diploidy gynogenesis of larvae are different from normal ones in insemination and germ plasm. We studied the effects of starvation on the growth and development of gynogenesis of turbot larvae by detecting the absorption of yolk and oil globule, the first feeding rate, the point of no return (PNR), and the growth and survival rate. During the experiment, the yolk and oil globules of starved gynogenetic larvae were completely disappeared on Day 6 and Day 7 after hatching, respectively. The highest feeding rate of starved larvae was about 85.3% occurred on Day 4, and PNR shown on Day 8. The development stopped and decreased on Day 6 onwards with syndromes of pectoral angle and body atrophy with color darkening. However, no distinct difference was found between starved gynogenetic larvae and the starved normal ones in absorption of yolk and oil globule, the first feeding rate, PNR, and the development length. Starvation did not affect the absorption of yolk and oil globule, which is the same as fed larvae. The consumption of yolk was centralized, while that of oil globule was uniform and mild. The starvation PNR arrived after inner source nutrition in larvae was used up. Feeding rate reached its peak during telophase of turbot larvae yolk absorption. These results are of significance for the study of larvae’s energy metabolism and growth.
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| Key words: Turbot Scophthalmus maximus, Diploidy gynogenesis of larvae, Starvation, Point of no return (PNR) |
