| 摘要: |
| 对我国沿海5 个自然群体长蛸的线粒体细胞色素氧化酶亚基I(COI)部分序列进行了测定和分析, 经比对获得658bp 核苷酸片段, 其中碱基T、C、A 和G 的平均含量分别为37.02%、18.46%、30.15%和14.35%, AT 的含量明显高于GC 的含量。5 个自然群体的长蛸中共发现18 个变异位点, 得到15 个单倍型, 包括4 个共享单倍型, 其中青岛群体的核苷酸差异数K 以及平均核苷酸多样性指数Pi 最高, 烟台群体最低; 群体间, 青岛群体在群体间核苷酸差异数K、群体间平均每位点核苷酸替代数Dxy 和群体间每位点净核苷酸替代数Da 三个指标上都表现出比其它群体之间较高的水平, 说明青岛群体具有最为丰富的遗传多样性。MEGA 3.1 软件计算5 个群体间的Kimura 2-paramter 遗传距离,大连群体和青岛群体之间的遗传距离最远为0.0077, 而大连群体与烟台群体之间的遗传距离最低,为0.0029。AMOVA 分析表明, 五群体间总遗传分化系数Fst = 0.17410 (P<0.05), 群体间遗传分化远小于群体内。NJ 法和UPGMA 法构建的分子进化树, 5 个地理群体的长蛸聚为两个族群, 大连、烟台群体聚为一族群, 青岛、连云港和舟山群体聚为另一族群。
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| 关键词: 长蛸, COI 基因, PCR, 遗传多样性 |
| DOI：10.11693/hyhz201002015015 |
| 分类号: |
| 基金项目:山东省优秀中青年科学家奖励基金(博士基金), 2006BS06010 号; 国家“863”计划“中国近海贝类资源的采集、保存与信息化技术”, 2007AA09Z433 号。 |
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| SEQUENCE AND MOLECULAR PHYLOGENY OF MITOCHONDRIAL COI GENE FRAGMENT IN FIVE POPULATIONS OF OCTOPUS VARIABILIS IN CHINA |
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SUN Bao-Chao1,2, YANG Jian-Min1,2, SUN Guo-Hua1, LIU Xiang-Quan1, LIU Li-Juan1, WANG Wei-Jun1, ZHENG Xiao-Dong3
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1.Shandong Marine Fisheries Research Institute;2.Shanghai Ocean University;3.Ocean University of China
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| Abstract: |
| Mitochondrial COI gene fragments of wild Octopus variabilis were amplified with PCR; the PCR products were purified and sequenced, and 658bp nucleotide sequences were obtained. The T, C, A, and G contents in this fragment were 37.02%, 18.46%, 30.15%, and 14.35%, respectively. The AT content was higher than the GC content. 18 variable nucleotide positions were detected in 658bp COI sequences analyzed; 15 haplotypes are defined among five populations, of which only 4 were common. Among the five populations, Qingdao (QD) shows the best genetic diversity. The Kimura 2-paramter genetic distance calculated by the MEGA version 3.1 between the population of Dalian (DL) and QD is the highest, up to 0.0077, while that between the populations of Dalian (DL) and Yantai (YT) is the lowest at 0.0029. The fixation indices (Fst) analyzed by AMOVA of ARLEQUIN Version 3.11 totaled to 0.17410 (P<0.05), showing that the genetic fixation intra-populations is much larger than that inter-populations. The Neighbor-joining (NJ) phylogenetic tree and UPGMA phylogenetic tree were made from the five populations of O. variabilis. The result shows that the population of DL, YT might have originated from one clade, while population of QD, LY and ZS from another.
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| Key words: Octopus variabilis, COI gene, PCR, Genetic diversity |
