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一株分离于湘江污染水域的酵母(Candida fermentati)的鉴定及其Cd2+的吸附特性
高 健, 邓先余, 康 健, 王志勇
湖南科技大学生命科学学院
摘要:
对湘江湘潭段周边污染水域的微生物群落结构进行调查, 筛选出一株能够耐受较高浓度镉的野生型酵母样真菌, 命名为XTWJX 菌株, 并对其进行形态学特征、生理试验以及ITS 序列和26SrDNA D1/D2 区序列分析。结果表明, 该菌株属于假丝酵母属的发酵假丝酵母Candida fermentati。金属抗性试验表明, XTWJX 菌株对测试的9 种不同金属离子显示出较高的抗性能力, 尤其是对毒性较大的Cd2+的抗性能力高达6mmol/L, 暗示该菌株可能具有应用前景。研究了XTWJX 菌株对Cd2+的吸附特性, 结果表明, 在Cd2+浓度为10mg/L 的水溶液中, 该菌株对Cd2+显示出有较强的吸附能力。这一结果暗示XTWJX 菌株的分离很可能为Cd2+污染的河流和湖泊等水生环境的生物修复提供了一种新型的生物材料。
关键词:  发酵假丝酵母, 形态学特征, 生理试验, 序列分析, 金属抗性, Cd2+吸附特性
DOI：10.11693/hyhz201003005005
分类号:
基金项目:湖南省教育厅资助项目, 09C378 号
IDENTIFICATION OF CADMIUM-ABSORBING STRAIN OF CANDIDA FERMENTATI FROM A POLLUTED AREA IN THE XIANGJIANG RIVER IN XIANGTAN
GAO Jian, DENG Xian-Yu, KANG Jian, WANG Zhi-Yong
School of Life Sciences, Hunan University of Science and Technology
Abstract:
A Cd2+-resistant strain of wild yeast-like fungus in polluted rivers and lakes was isolated in Xiangjiang River near Xiangtan City, named by strain XTWJX. Researches in morphology, physiology, and molecular biology (ITS and 26S rDNA D1/D2) were conducted. Result shows that it belongs to Candida fermentati, could survive in high cadmium concentration of 6.0mmol/L, and could adsorb cadmium in 10mg/L cadmium solution. Therefore, the strain may be applied potentially as a new biomaterials for treatment of cadmium-polluted water.
Key words:  Candida fermentati, Morphological characteristics, Physiological test, Sequence analysis, Metal resistance, Cadmium absorption
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