| 摘要: |
| 采用静水法生物测试手段, 研究了不同浓度梯度Cu、Pb(各0.00、0.10、0.25、0.50mg/L)随时间变化在泥鳅卵巢中的蓄积特性以及对卵细胞发育的影响。结果表明, 泥鳅卵巢对Cu、Pb 有较强的蓄积能力, 在暴露期间20 天内, 蓄积量与时间呈线性关系, 之后呈对数关系。其中, Cu 的蓄积显著大于Pb (P<0.01); 卵巢中重金属的蓄积与水溶液中Cu、Pb 的浓度梯度相关(P<0.01), 具有明显的时间效应和剂量效应。泥鳅卵巢中卵细胞的发育水平, 亦与溶液重金属浓度的高低、暴露时间长短有关; 在不同浓度重金属溶液中较长时间的暴露, 将对卵巢及卵细胞的发育产生不可逆转的负效应。
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| 关键词: Cu, Pb, 泥鳅, 卵巢, 蓄积特性, 卵细胞发育 |
| DOI：10.11693/hyhz201003014014 |
| 分类号: |
| 基金项目:浙江省自然科学基金项目, Y307535 号, Y304185 号; 浙江省教育厅科研计划项目, Y200804870 号 |
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| ACCUMULATION OF HEAVY METALS (Cu2+ AND Pb2+) IN THE OVARY OF MISGURNUS ANGUILLICAUDATUS AND THE SUBSEQUENT EFFECTS ON OVA DEVELOPMENT |
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TANG Jian-Xun, XING Cheng-Hua, LIU Zhong-Liang, CHENG Zhang-Shun, LI Jun-Rong
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Agricultural and Bio-Engineering School, Jinhua College of Vocation and Technology
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| Abstract: |
| We studied the accumulation of Cu2+ and Pb2+ (0.00, 0.10, 0.25 and 0.50mg/L, respectively) in the ovary of Misgurnus anguillicaudatus as well as the subsequent effects on ova development. We found that the ovarian accumulation
of Cu2+ and Pb2+ was time- dose- dependently related to the environmental concentrations (P<0.01). The ovary of M. anguillicaudatus showed a high capacity of accumulating Cu2+ and Pb2+; the correlation between the accumulation level and the exposure time for the initial 20 days was linear, and became exponential afterwards. In addition, the accumulation of
Cu2+ was significantly higher than that of Pb2+ (P<0.01). The development of the ova was affected by the concentrations and exposure duration; prolonged exposure to various concentrations of these heavy metals resulted in irreversible damage in the ovaries and the ova.
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| Key words: Cu, Pb, Misgurnus anguillicaudatus, Ovary, Accumulation characteristic, Ovum development |
