| 摘要: |
| 采用序列相关扩增多态SRAP(Sequence-related amplified polymorphism)标记对国家级江苏文蛤良种场(筹)保存的广西北海(GX)、江苏南通(JS)、辽宁大连(LN)三个地理种群原种文蛤进行种质资源分析。结果表明: (1) 15 对引物在文蛤3 种群共扩增223 个位点, 其中多态位点数为159 个, 多态性位点百分率为71.30%, 显示了较高的多态性; (2) GX、JS、LN 3 种群的多态性位点百分率分别为69.12%、57.81%、51.85%, 平均杂合度分别为0.2532、0.2162、0.1836, 香农信息指数分别为0.3649、0.3127、0.2714, 均以GX 种群最高, 表明GX 种群遗传多样性更为丰富; (3) 3 种群扩增位点显性基
因频率的分布规律揭示, 与LN 和JS 两种群相比, GX 种群中低频基因位点显著增加, 而隐性纯合基因位点显著减少, 表明GX 种群中存在大量稀有等位基因。各地理种群原种文蛤遗传多样性水平相对较高, 遗传改良潜力大, GX 种群作为选择育种的基础群更为适合。
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| 关键词: 文蛤, 种质资源, SRAP |
| DOI：10.11693/hyhz201003021021 |
| 分类号: |
| 基金项目:江苏省水产三项更新工程项目, K2007-7 号、BZ2008-9 号; 江苏省自然科学基金项目, BK2008191 号。 |
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| SRAP ANALYSIS ON GERMPLASM OF WILD MERETRIX MERETRIX OFF CHINESE COASTS |
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ZHANG Zhi-Wei1, CHEN Ai-Hua1, YAO Guo-Xing1, WU Jian-Ping1, WU Yang-Ping1, XU Guang-Ping1, CHENG Han-Liang2
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1.Jiangsu Institute of Marine Fishery;2.College of Marine Science, Huaihai Institute of Technology
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| Abstract: |
| Genetic variation of three wild stocks of Meretrix meretrix was analyzed using sequence-related amplified polymorphism (SRAP) markers. Samples were collected in Guangxi (GX), Jiangsu (JS), and Liaoning (LN) provinces and kept in Hard Clam National Breeding Plant in China. Fifteen primer pairs produced 159 polymorphic loci in average of 10.6 loci per primer pair. The percentage of polymorphic loci was 71.30%, suggesting a rich genetic diversity among the wild resources. The percentage of polymorphic loci in Populations GX, JS, and LN was 69.12%, 57.81%, and 51.85%; the average heterozygosis of population was 0.253, 0.216, 0.184; and the Shannon-Wiener index was 0.365, 0.313, 0.271, respectively. The results indicate that Population GX had the highest genetic diversity among three populations, and a large amount of low frequency alleles indicated by noticeable increase in the number of rare loci and decrease in the number of homozygous recessive loci; therefore, the population is potential for genetic improvement of breed.
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| Key words: Meretrix meretrix, Germplasm, SRAP |
