| 摘要: |
| 甲壳动物的卵巢发育及卵黄发生受到体内各种内分泌激素的调控。本文研究了两组不同饲料—脂类营养平衡组(A组, 含有磷脂和HUFA)和不平衡组(B组, 缺乏磷脂和HUFA)对中华绒螯蟹卵巢发育及卵黄发生的激素调控, 运用放射免疫法和放射化学法分别测定了处于不同卵黄发生期的中华绒螯蟹的肝胰腺、卵巢、血淋巴中孕酮(PG)、雌二醇(E2)的含量及中华绒螯蟹大颚器(MO)合成与分泌甲基法尼酯(MF)的速率。结果表明: 1) 中华绒螯蟹体内三种组织(肝胰腺、卵巢和血淋巴)中均存在 PG和 E2, PG可能为 E2 的前体; 2) 早熟蟹的 MO合成 MF速率远远大于正常发育的幼蟹, MF的大量合成与分泌是促使早熟蟹产生的主要内分泌因素; 3) B 组饲料(含猪油)喂养的河蟹其 MO 合成MF 的速率均高于 A 组饲料(含鱼油), 且 B 组饲料的早熟蟹转化 PG 速度比 B 组饲料的早熟蟹快; 4) PG和E2的脂类结合部位能结合外源性卵黄蛋白原(Vg), 通过血淋巴的运输被卵泡细胞胞吞, 最终与卵母细胞内的内源性 Vg 结合, 共同形成卵黄体; 5) 多不饱和脂肪酸(如 EPA 和 DHA)可能会抑制类固醇激素的产生。 研究表明, MF是中华绒螯蟹的促性腺激素, 具有促使PG合成和E2转化的作用, 它们共同诱导河蟹卵巢发育及卵黄发生。
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| 关键词: 中华绒螯蟹, 卵巢发育, 卵黄发生, 早熟, 孕酮, 雌二醇, 甲基法尼酯 |
| DOI：10.11693/hyhz201004006006 |
| 分类号: |
| 基金项目:国家自然科学基金项目, 30901110号、 30471349号; 上海市农委攻关项目, 沪农科推字(2008)第2-4号、 (2009)第2-1号; 上海高校创新团队(第二期)资助, 2010—2012; 国家农业科技成果转化基金项目, 2009GB2C300156 号 |
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| HORMONAL REGULATIONS OF OVARIAN DEVELOPMENT AND VITELLOGENESIS IN CHINESE MITTEN CRAB ERIOCHEIR SINENSIS FED ON TWO DIFFERENT DIETS |
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LU Jian-Feng1,2, CHANG Guo-Liang3, WU Xu-Gan2, YANG Xiao-Zhen2, ZHAO Wei-Xin2, CHENG Yong-Xu2
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1.School of Biotechnology and Food Engineering, Hefei University of Technology;2.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,;3.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,School of Life Sciences, Huaiyin Normal University
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| Abstract: |
| crustaceans, ovarian development and vitellogenesis are known to be regulated by circulating hormones. This study examined the hormonal regulations of ovarian development and vitellogenesis in Chinese mitten crab Eriocheir sinensis fed on two different diets (group A, lipid nutrition balance; group B, lipid nutrition unbalance). The concentrations of progesterone (PG) and estradiol (E2) in the hepatopancreas, ovary, and hemolymph of E. sinensis were measured at different vitellogenic stages by radioimmunoassay. Methyl farnesoate (MF), biosynthesized and secreted by mandibular organ (MO), were analyzed by radiochemical method. The main results were as follows: 1) Occurrence of PG and E2 in the hepatopancreas, ovary and hemolymph, and PG might be the precursor of E2; 2) MF biosynthesis rates of precocious crabs were markedly higher than those of normal juvenile crabs, and large amounts of MF biosynthesized and secreted by MO were the endocrine basis for precocity; 3) MF biosynthesis rates of group B (diets containing pork lard ) were higher than those of group A (diets containing fish oil and soy lecithin), and conversion rates of PG to E2 of group B were also higher than those of group B; 4) PG and E2 possibly bound to Exogenous vitellogenin (Vg) at their lipid binding sites, and were then transported via haemolymph to follicle cells via receptor-mediated endocytosis, and finally integrated with endogenous Vg of oocytes, resulting in their incorporation into the yolk; 5) PUFA such as EPA and DHA might inhibit the production of steroid hormone. Results of this study indicated that MF might act as a gonadotropin which stimulated PG synthesis and E2 conversion, and these three hormones together induced ovarian development and vitellogenesis.
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| Key words: Eriocheir sinensis, Ovarian development, Vitellogenesis, Precocity, Progesterone, Estradiol, Methyl farnesoate |
