| 摘要: |
| 以南极海洋细菌为研究对象, 采用 2216E 和 MMC 液体培养基结合的方法, 从菌种库里筛选南极低温降解菌, 进行石油烃降解率测定、 表型特征确定和 16S rRNA 分子鉴定, 构建系统发育树, 并对南极低温降解菌 NJ49 的低温降解适应性进行了初步研究。结果表明, 3 株南极海洋细菌 NJ41、NJ49 和 NJ289 可以在以柴油为惟一碳源和能源的培养基中生长, 被视为南极低温降解菌; 16S rRNA分子鉴定结果表明, 南极低温降解菌 NJ41、NJ49 和 NJ289 分别属于 Planococcus、Shewanella 和 Pseudoalteromonas 属; NJ49 通过改变脂肪酸组分的膜修饰方式适应低温降解。
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| 关键词: 极海洋, 降解菌, 柴油, 16S rRNA, 低温适应性 |
| DOI：10.11693/hyhz201005005005 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目, 40876107 号; 国家海洋局青年海洋科学基金项目, 2010122 号 |
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| SCREENING, IDENTIFICATION OF ANTARCTIC COLD-ADAPTED PETROLEUM HYDROCARBON-DEGRADING BACTERIA AND LOW-TEMPERATURE DEGRADING ADAPTATION ANALYSIS |
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LIU Fang-Ming1,2, MIAO Jin-Lai1,2, DONG Chun-Xia3, WANG Yi-Bin1,2, ZANG Jia-Ye1
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1.First Institute of Oceanography, State Oceanic Administration;2.Key Lab of Marine Bioactive Substances, State Oceanic Administration;3.Estong (Qingdao) Industry Company, Ltd.
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| Abstract: |
| Low temperature microorganism resources of Antarctica were abundant and the scholar abroad have focused on obtaining cold-adapted degrading bacteria. The method of combination 2216E with MMC culture was used for screening petroleum hydrocarbon-degrading cold-adapted bacteria which were from Antarctic seawaters . Degradation efficient, molecular identification of 16S rRNA amplified from four Antarctic cold-adapted degrading bacteria and phylogenetic tree based on 16S rRNA gene was carried. Antarctic cold-adapted degrading bacteria NJ49 was chosen to study cold adaptation. The results suggested that three marine bacteria cold-adapted bacteria (namely NJ41, NJ49, NJ289) grew well in the culture which diesel was the sole carbon source and energy source. Results of 16S rRNA identification revealed that Antarctic cold-adapted degrading bacteria NJ41, NJ49 and NJ289 belonged to the described genus Planococcus, Shewanella, Pseudo-alteromonas, respectively. Data of fatty acid change revealed that the Antarctic cold-adapted degrading bacteria NJ49 maybe adapt cold condition to degrade by membrane modification of changing fatty acid.
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| Key words: tarctic sea, degrading bacteria, Diesel, 16S rRNA, Low-temperature adaptation |
