| 摘要: |
| 采用聚丙烯酰胺凝胶电泳技术对半滑舌鳎野生和养殖 4 个群体的遗传结构进行了比较研究。结果表明, 半滑舌鳎养殖群体遗传变异水平低于野生群体, 两个野生群体的多态位点百分数、观察杂合度、期望杂合度、平均有效等位基因数的平均值(分别为 21.275%、0.1177、0.0854 和 1.1499)分别高于两个养殖群体的平均值(分别为 18.75%、0.1156、0.0741 和 1.1355)。渤海与黄海野生群体间遗传分化较低, 唐山野生群体与江苏连云港野生群体间遗传距离为 0.0006, 群体间分化较低(FST = 0.0109, Nm = 22.5850)。
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| 关键词: 半滑舌鳎, 等位酶, 遗传结构, 遗传变异, 遗传分化 |
| DOI：10.11693/hyhz201101017017 |
| 分类号: |
| 基金项目:福建省科技重点项目“半滑舌鳎南方产业化人工育苗技术开发及养殖示范”, 2008N0039 号; 福建省海洋与渔业局“名特优新海水鱼人工繁育和健康养殖规模化开发技术研究”, 200805150006 号; 集美大学创新团队基金, 2010A004 号; 福建省高等教育新世纪优秀人才支持计划, 闽教科(2006)35 号。 |
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| THE GENETIC STRUCTURES OF WILD AND CULTIVATED POPULATIONS OF CYNOGLOSSUS SEMILAEVIS |
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CHANG Jian-Bo1,2, LEI Guang-Gao1,2, LI Zhong-Bao1,2, ZHAO Bin-Li1,2, CHEN Jin1,2, ZHANG Gui-Ling1,2, WANG Zhan-Lin1,2
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1.Fishery College, Jimei University;2.Institute of Aquaculture Biotechnology, Jimei University
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| Abstract: |
| Genetic structures of wild and cultivated populations of Cynoglossus semilaevis were investigated using the assay of vertical slab polyacrylamide gel electrophoresis. The results showed there were loss of genetic diversity in cultivated populations of C. semilaevis compared with wild populations, the average of percentage of polymorphic loci, the average of observed heterozygosity, the average of expected heterozygosities, and the mean effective number of alleles per locus of wild C. semilaevis populations (21.275%, 0.1177, 0.0854 and 1.1499, respectively) were all higher than those of cultivated C. semilaevis populations (18.75%, 0.1156, 0.0741 and 1.1355, respectively). The genetic differentiation between wild C. semilaevis populations was low, genetic distance, genetic differentiation and gene flow between populations were 0.0006, 0.0109 and 22.5850, respectively.
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| Key words: Cynoglossus semilaevis, Allozyme, Genetic structure, Genetic variation, Genetic differentiation |
