| 摘要: |
| 使用气升式光生物反应器培养钝顶螺旋藻 25天, 发现随螺旋藻生物量的增长, 细菌生物量有一定的增长趋势。应用 PCR-DGGE 技术分析螺旋藻培养过程中细菌种类组成, 发现在不同时期细菌群落组成变化明显。通过 DGGE 图谱中 18 条特征条带的克隆、测序及系统进化分析, 发现其中有8条序列与α变形菌纲序列相似, 3条与拟杆菌纲序列相似, 2条与γ变形菌纲序列相似, 2条与异常球菌纲序列相似, 2 条与放线菌纲相似, 还有 1 条与蓝藻纲的序列相似。在所有相似序列中, 有 17 条来源于高温、碱性、淡水环境, 与已报道的螺旋藻的培养环境相似。研究发现α变形菌纲与拟杆菌纲的细菌为整个培养过程中的优势类群。
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| 关键词: 钝顶螺旋藻, 细菌, 气升式反应器, PCR-DGGE, 16S rDNA 序列分析 |
| DOI：10.11693/hyhz201101023023 |
| 分类号: |
| 基金项目:国家 “863” 项目资助, 2007AA09Z434号; 国家908专项, 908-01-ZH3号; 中国科学院创新项目, KZCX2-YW-213-3 |
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| BACTERIAL COMMUNITY VARIATION OF SPIRULINA PLARENSIS CULTURED IN AIRLIFT REACTOR |
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DONG Yi1,2, LIU Min1,2, WANG Jin-Xia3, XIAO Tian1
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1.Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences;2.Graduate School, Chinese Academy of Sciences;3.Institute of Oceanology, Chinese Academy of Sciences
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| Abstract: |
| Using airlift reactor to cultivate Spirulina plarensis for 25 days, bacterial biomass increased with the increase of the biomass of S. plarensis. The bacterial community composition of different cultural periods changed significantly. Eighteen DGGE bands were excised, cloned and sequenced. Among these sequences, 8 were similar to α-Proteobacteria, 3 were similar to Bacteroidetes, two were similar to γ- Proteobacteria, two were similar to Deinococci, two were similar to Actinobacteria, and 1 was similar to Cyanobacteria. There were 17 sequences rooted in the similar environment of the S. plarensis culture. During the culture, ?-Proteobacteria and Bacteroidetes were the superior genera.
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| Key words: Spirulina, Bacteria, Airlift reactor, PCR-DGGE, 16S rDNA sequence analysis |
