| 摘要: |
| 菱形藻是较常见的羽纹硅藻类, 其中一些种类的外部形态十分相似, 差异细微, 很难从经典形态分类的角度准确区分, 造成此类微藻在分类地位上的混乱。作者综合利用经典形态分类方法、ITS(Internal transcribed spacer sequences)序列分析方法以及基于脂肪酸甾醇的化学分类多种方法, 对4 株从形态观察初步确定为菱形藻及其近缘种类的野外采集样品以及在分类上仍存分歧的小新月菱形藻(Nitzschia closterium f. minutissima)和三角褐 |
| 关键词: 菱形藻, 近缘种, 分类, 比较研究 |
| DOI：10.11693/hyhz201103003003 |
| 分类号: |
| 基金项目:国家自然科学基金项目, 40776064 号; 教育部长江学者与创新团队项目, IRT0734 号; 教育部博士点基金项目, 200816460002号; 宁波市重大科技项目, 2006C100030号; 宁波科技计划项目, 2007A31004号。 |
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| A COMPARATIVE STUDY ON THE IDENTIFICATION OF NITZSCHIA-RELATED SPECIES |
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LIU Bao-Ning, LIU Chen, ZHOU Cheng-Xu, WU Xiao-Kai, SUN Xue, XU Ji-Lin
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Key Laboratory of Applied Marine Biotechnology (Ningbo University), Ministry of Education
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| Abstract: |
| Nitzschia spp. belongs to a common group of pennate diatom. Some species in this genus have subtle morphological differences, which make them difficult to be properly identified based on their morphological profiles only. In this paper, three taxonomical methods, namely microscopy observation, internal transcribed spacer sequence (ITS) sequence analysis, and microalgal fatty acids and sterols analysis, were used in the study of four field samples, which were preliminary identified to be Nitzschia genus and two closely related species, i.e., Nitzschia closterium f. Minutissima and Phaeodactylum tricornutum, using morphological method. We found that more information could be obtained by applying multiple methods in identifying microalgal strains than using only one method. Similar taxonomic results were obtained using these three methods. From the study, three field samples were identified to be Pseudo-nitzschia pungens Hasle, Pseudo-nitzschia delicatissima, and Cylindrotheca closterium, respectively. However, we were only able to identify to the fourth strain as Cylindrotheca genus. The results also showed that Nitzschia closterium f. Minutissim belongs to Naviculales, Phaeodactylaceae, Phaeodactylum, consistent with previous findings. |
| Key words: Nitzschia related species taxonomy comparative study |
