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| 半滑舌鳎(Cynoglossus semilaevis)野生与养殖群体遗传多样性的比较研究 |
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黎中宝1,2, 雷光高1,2, 常建波1,2, 赵斌丽1,2, 陈 锦1,2, 张桂玲1,2, 王展林1,2
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1.集美大学水产学院;2.集美大学水产生物技术研究所
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| 摘要: |
| 采用扩增片段长度多态性技术(AFLP技术)对2个野生和2个养殖的半滑舌鳎群体进行了遗传多样性的比较研究。实验采用 8 对 AFLP 引物组合, 在四个群体的 120 个个体中共扩增出 498 个位点。结果表明, 半滑舌鳎养殖群体的遗传多样性降低: 河北唐山野生群体和江苏连云港野生群体的多态位点百分数分别为 45.18%和39.96%, Nei遗传多样性指数分别为 0.1159和 0.1059, Shannon多样性指数分别为 0.1817 和 0.1663, 平均有效等位基因数分别为 1.1884 |
| 关键词: 半滑舌鳎, AFLP, 群体, 遗传多样性 |
| DOI：10.11693/hyhz201103012012 |
| 分类号: |
| 基金项目:福建省科技重点项目“半滑舌鳎南方产业化人工育苗技术开发及养殖示范”, 2008N0039 号; 福建省海洋与渔业局“名特优新海水鱼人工繁育和健康养殖规模化开发技术研究”, 200805150006 号; 集美大学创新团队基金, 2010A004 号; 福建省高等教育新世纪优秀人才支持计划, 闽教科(2006)35 号 |
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| COMPARISON OF GENETIC DIVERSITY OF WILD AND CULTIVATED POPULATIONS OF CYNOGLOSSUS SEMILAEVIS |
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LI Zhong-Bao1,2, LEI Guang-Gao1,2, CHANG Jian-Bo1,2, ZHAO Bin-Li1,2, CHEN Jin1,2, ZHANG Gui-Ling1,2, WANG Zhan-Lin1,2
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1.Fishery College, Jimei University;2.Institute of Aquaculture Biotechnology, Jimei University
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| Abstract: |
| Genetic diversity of wild and cultivated populations (4 populations) of Cynoglossus semilaevis were analyzed using the AFLP method. A total of 498 loci were detected from 120 individuals using eight AFLP primer combinations. The results from AFLP technique showed that there was a loss of the genetic diversity in cultivated populations of C. semilaevis compared to wild populations. The percentages of polymorphic loci, the Nei’s gene diversity, Shannon’s information index and the mean effective number of alleles per locus in two wild populations were 39.96%—45.18%, 0.1059—0.1159, 0.1663—0.1817 and 1.1704—1.1884, respectively; in contrast, those of the two cultivated populations were 37.75%—37.95%, 0.1015—0.1046, 0.1587—0.1629 and 1.1635—1.1696, respectively. Genetic distance among the 4 populations varied from 0.0648—0.0820 with a mean of 0.0725. |
| Key words: Cynoglossus semilaevis, AFLP, Population, Genetic diversity |