| 摘要: |
| 利用 AFLP 技术分析了我国沿海棘头梅童鱼 8 个地理群体(山东荣成、江苏南通、启东、上海芦潮港、浙江温州、瑞安、福建福州和广州番禺)的遗传结构特征。10 对引物组合在 8 个群体 160尾个体中共扩增出 272 个位点, 多态位点 72 个, 多态位点比例为 26.47%。8 个地理群体的多态位点比例、Nei 遗传多样性指数分别为 8.82%—15.07%、0.0291—0.0514, 表明棘头梅童鱼群体遗传多样性水平较低。AMOVA 分析表明, 群体间差异对群体遗传总变异的贡献率为 47.9 |
| 关键词: 棘头梅童鱼, 群体, 遗传结构, AFLP |
| DOI：10.11693/hyhz201103017017 |
| 分类号: |
| 基金项目:上海市科技兴农重点攻关项目资助, 沪农科攻字(2007)第4-2号; 上海市重点学科建设项目资助, Y1101号 |
|
| GENETIC STRUCTURE OF COLLICHTHYS LUCIDUS POPULATIONS FROM CHINA COASTAL AREAS BY AFLP ANALYSIS |
|
ZHENG De-Feng1, ZHAO Jin-Liang1, ZHOU Wen-Yu2, YAN Jun-Cong1
|
|
1.Key Laboratory of Aquatic Genetic Resources and Utilization Ministry of Agriculture, Shanghai Ocean University;2.Shanghai Fisheries Research Institute
|
| Abstract: |
| The genetic structure of Collichthys lucidus populations from eight China coastal areas (Rongcheng, Nantong, Qidong, Luchaogang, Ruian, Wenzhou, Fuzhou and Panyu) was analyzed by amplified fragment length polymorphism (AFLP) technique. A total of 272 loci were generated with 10 primer combinations in 160 individuals of the eight populations, of which 72 (26.47%) were polymorphic loci. The results showed the genetic diversity of C. lucidus was relatively low, the proportion of polymorphic loci and Nei's genetic diversity index for eight populations were 8.82%—15.07% and 0.0291—0.0514, respectively. AMOVA showed the variance of intra-populations (52.09%) was similar to that of inter-populations (47.91%), and there were significant differences among inter-populations and intra-populations. The genetic divergence index Fst values among eight populations were in the range of 0.1151—0.5995 (P<0.05), significant differentiation existed among different populations. UPGMA dendrogram showed that Rongcheng and Nantong populations formed the first clade, Qidong, Luchaogang and Ruian populations formed the second clade, and Wenzhou, Fuzhou and Panyu populations formed the third clade. Though south-north divergence was observed among the C. lucidus populations, there was no distinct boundary for these clades because of gene exchanges. |
| Key words: Collichthys lucidus, Population, Genetic structure, Amplified fragment length polymorphism (AFLP) |
