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组织研磨器细胞破碎法提取霞水母(Cyanea sp.)毒素的初步研究
李荣锋1,2, 于华华1, 冯金华1, 李鹏程1
1.中国科学院海洋研究所;2.中国科学院研究生院
摘要:
采用 Mini-Beadbeater 组织研磨器破碎的方法进行了提取霞水母刺丝囊细胞毒素的研究。 首先制备霞水母刺丝囊细胞, 然后研究破碎频率、破碎时间对提取霞水母刺丝囊细胞毒素浓度的影响。结果表明, 组织研磨器能够在 2—3min 内破碎约 90%的霞水母刺丝囊细胞, 从而提高水母毒素的提取效率。 SDS-PAGE 结果显示, 提取的水母毒素蛋白条带没有发生明显差异, 是一种有效提取霞水母毒素的方法。
关键词:  组织研磨器, 霞水母, 刺丝囊细胞, 毒素提取
DOI：10.11693/hyhz201106007007
分类号:
基金项目:国家自然科学基金项目, 41006095号; 国家科技支撑计划，2011BAE06B04-05号; 青岛市科技计划联合基金项目, 08-1-3-51-jch号; 中国科学院海洋研究所知识创新工程青年人才领域前沿项目, 2009—2011
METHODS OF EXTRACTING VENOM FROM JELLYFISH CYANEA SP. NEMATOCYST USING MINI-BEADBEATER
LI Rong-Feng1,2, YU Hua-Hua1, FENG Jin-Hua1, LI Peng-Cheng1
1.Institute of Oceanology, Chinese Academy of Sciences;2.Graduate School, Chinese Academy of Sciences
Abstract:
Extraction of venom from jellyfish Cyanea sp. nematocysts using Mini-Beadbeater under different conditions was investigated. First, jellyfish nematocysts were prepared from tentacles, then concentration of nematocyst venom extracted in different disruption conditions, such as disruption time and power, were determined. Microscopy images of disrupted jellyfish nematocysts showed only taken 2—3min for Mini-Beadbeater to disrupt approximately 90% of the nematocysts, an improvement in yield in extracting venom in jellyfish. The protein profile showed no obvious difference from our SDS-PAGE results. Our extraction methodology is proven to be an effective way to extract the venom from jellyfish Cyanea sp.
Key words:  Mini-Beadbeater, Cyanea sp., Nematocyst, Venom extraction
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