| 摘要: |
| 利用 ISSR 分子标记技术获得长蛸的 ISSR-PCR 最优反应体系, 并对大连、烟台、青岛、连云港、舟山、温州、厦门 7 个群体 168 个个体进行遗传多样性分析。结果表明, 7 个引物在 7 个群体中共扩增出 118 条重复性好且带型清晰的 ISSR 扩增带, 其中多态条带为 55 条, 多态性比例达46.61%。 在物种水平上的 Nei’s 基因多样性指数和 Shannon’s 信息指数分别为 0.3145 和 0.4766, 在群体水平上分别为 0.1573 和 0.2355。7 个群体可明显聚类为 2 个类群, AMOVA 分子变异分析发现, 长蛸 54.64541%的变异发生在群体间, 45.35459%的变异发生在群体内。群体间的遗传分化系数 Gst 为0.5067 和由 Gst 估算的基因流 Nm 为 0.4867<1。分析显示长蛸具有较高遗传多样性水平, 但群体间已经出现了较大程度的遗传分化。 |
| 关键词: 长蛸, 遗传多样性, ISSR |
| DOI：10.11693/hyhz201106019019 |
| 分类号: |
| 基金项目:国家高技术研究发展计划(863 计划)课题“名贵头足类苗种规模繁育关键技术”, 2010AA10A404 号; 国家海洋公益性行业科研专项, 201005013 号; 海洋渔业科学与技术浙江省重中之重学科开放课题, 20100105 号; 浙江海洋学院人才引进启动项目, 21135011509 号 |
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| GENETIC DIVERSITY OF DIFFERENT GEOGRAPHICAL POPULATIONS IN OCTOPUS VARIABILIS REVEALED BY ISSR ANALYSIS |
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GUO Bao-Ying, ZHOU Chao, Lü Zhen-Ming, LI Ji-Ji, WU Chang-Wen
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Zhejiang Ocean University, Key Laboratory of Mariculture Equipments and Engineering Technology of Zhejiang Province
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| Abstract: |
| In this study, genetic diversity among 168 individuals of 7 different geographical populations of the Octopus variabilis were analyzed after we had established the ISSR-PCR optimal reaction system by the Inter-Simple Sequence Repeat (ISSR) markers. The samples of the seven populations were taken from Dalian, Yantai, Qingdao, Lianyungang, Zhoushan, Wenzhou and Xiamen, respectively. Under predetermined optimal reaction conditions, total of 118 reproducible and clear DNA fragments from seven populations were amplified with seven primers, in which 55 fragments were polymorphic, and the proportion of polymorphic loci was 46.61%. The Nei’s gene diversity and Shannon’s information index of the O. variabilis was 0.3145 and 0.4766 at species level, 0.1573 and 0.2355 at population level, respectively. 7 populations could be apparently divided into 2 groups. The AMOVA demonstrated that the among-population component accounted for 54.64541% of the total variation, while the within-population component accounted for 45.35459%. The genetic differentiation coefficient (Gst) and the gene flow (Nm) were 0.5067 and 0.4867 (< 1), respectively among the 7 populations. These data indicate that the genetic diversity of O. variabilis is relatively high, and there is genetic differentiation of some extent among the seven populations of O. variabilis. |
| Key words: Octopus variabilis, Genetic diversity, ISSR |
