| 摘要: |
| 采用AFLP技术和单因素方差分析方法, 进行了青蛤主要生长性状相关分子标记筛选研究。结果表明, 检测的 18 个与壳长相关的基因位点, 其贡献率在 6.46%—18.32%之间, 其中 E32M51-8、E33M51-38 对壳长的贡献率较高, 分别为 18.32%和 15.68%。检测的 23 个与壳宽相关的基因位点, 其贡献率在 6.75%—20.93%之间, 其中 E32M51-8、 E33M48-19 对壳宽的贡献率较高, 分别为 20.93%和17.34%。 检测的 22 个与壳高相关的基因位点, 其贡献率在 6.54%—15.91%之间, 其中 E32M51-17 对壳高的贡献率最高, 为 15.91%。检测的 8 个与活体重相关的基因位点, 其贡献率在 6.65%—17.83%之间, 其中 E33M48-10 对活体重的贡献率最高, 为 17.83%。以上结果说明青蛤生长性状的遗传基础复杂, 通过筛选与其相关显著的分子标记, 在育种中开展分子标记选择是可行的。 |
| 关键词: 青蛤, 生长性状, AFLP 标记, 贡献率 |
| DOI：10.11693/hyhz201201018018 |
| 分类号: |
| 基金项目:浙江省自然科学基金项目, Y3090007 号; 浙江省科技厅公益性应用技术研究计划项目, 2009C32020 号; 台州市科技计划项目, 08KY07 号; 浙江省近岸水域生物资源开发与保护重点实验室开放课题基金, 2010F30003 号。 |
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| AFLP ANALYSIS OF MOLECULAR MARKERS RELATED TO GROWTH TRAITS IN CYCLINA SINENSIS |
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SUN Chang-Sen1, WANG Teng-Da2, DONG Ying-Hui2, YAO Han-Han2, LIN Zhi-Hua2
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1.School of Life Science, Taizhou University;2.College of Biological and Environmental Sciences, Zhejiang Wanli University
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| Abstract: |
| In this paper, 60 mixed breeding offsprings from two natural populations of Cyclina sinensis were used to screen molecular markers related to shell length, shell width, shell height and body weight under the method of AFLP and one-factor analysis of variance. The results showed: of detected 18 gene loci related to shell length, the contribution rates were between 6.46% and 18.32%, and the contribution rates of E32M51-8, E33M51-38 were higher, respectively 18.32% and 15.68%. The contribution rates of detected 23 gene loci associated with shell width were between 6.75% and 20.93%, and those of E32M51-8, E33M48-19 were higher, respectively 20.93% and 17.34%. For shell height, 22 associated gene loci were detected, their contribution rates were between 6.54% and 15.91%, and the E32M51-17 locus had the highest contribution rate. Eight gene loci related to body weight were analyzed, the highest contribution rate (locus E33M48-10) was 17.83%, and lowest value was 6.65%. |
| Key words: Cyclina sinensis, Growth traits, AFLP marker, Contribution rate |
