| 摘要: |
| 采用 PCR 扩增和序列测定等技术, 对脊尾白虾 3 个野生群体共计 58 个个体的线粒体 16S rRNA 基因片段进行初步研究。结果表明, 在获得的长度为 509bp 核苷酸序列中, 共检测到 7 个变异位点、8 种单倍型, 除象山湾群体外其它两个群体遗传多样性水平都较低。AMOVA 分析表明, 象山湾与莱州湾群体有显著的遗传差异, 其余群体间的遗传差异不显著。另外, 将本研究所得序列与GenBank 中长臂虾科 11 个种的 16S rRNA 基因片段序列进行比较后, 发现其聚类关系与传统分类略有不同, 并依据 16S rRNA 序列变异百分比推测了长臂虾科 12 个种的大致分化时间。 |
| 关键词: 脊尾白虾, 16S rRNA 基因, 遗传多样性, 系统进化 |
| DOI：10.11693/hyhz201201028028 |
| 分类号: |
| 基金项目:国家高技术研究发展计划项目资助, “主要养殖甲壳类良种培育”, 2012AA10A409 号; 山东省科技发展计划项目资助，2011GHY11526 号; 农业科技成果转化资金项目资助, 2010GB23260589 号 |
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| THE GENETIC DIVERSITY OF THE 16S rRNA GENE IN THE MITOCHONDRIAL DNA OF THREE WILD POPULATIONS OF EXOPALAEMON CARINICAUDA |
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MA Peng1,2, LIU Ping1, LI Jian1, LI Ji-Tao1, GAO Bao-Quan1
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1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;2.Ocean University of Dalian
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| Abstract: |
| Using PCR amplification and sequencing analysis, we examined the 16S rRNA gene in the mitochondrial gene of total 58 individuals that belonged to three wild populations of Exopalaemon carinicauda, which were sampled in the Haizhou, the Xiangshan, and the Laizhou Bays. 16S rRNA fragments that had 509bp were obtained in our study. Through comparing the variation sites, nucleotide diversity and average number of nucleotide differences were calculated to analyze the gene sequence variation and genetic diversity. The results showed that the colonies from the Haizhou and the Laizhou Bays had lower levels of genetic diversity than that from the Xiangshan Bay. Fst values between the Xiangshan and the Laizhou Bays showed significant genetic differences, and Fst values among all the other populations showed no strong genetic differences. The molecular phylogenetic tree was constructed using the NJ method to study molecular phylogeny of several species of Palaemonidae. Our finding indicated that the Phylogenetic tree clustered into two branches: Palaemoninae clustered into one branch, and Pontoniinae clustered into the other. In addition, differentiation time of 12 species of the Palaemonidae was deduced base on the percentage of the 16S rRNA divergence. |
| Key words: Exopalaemon carinicauda, 16S rRNA gene, Genetics diversity, Phylogenetic |
