| 摘要: |
| 分别以高氮(摩尔C/N=8.5)、低氮(C/N=12.7)和氮添加(C/N=9.6)亚心形四爿藻(Tetraselmis subcordiformis)喂食安氏伪镖水蚤(Pseudodiaptomus annandalei)的幼体和成体, 研究了此水蚤在不同氮条件下的生长、发育和摄食。随着藻细胞C/N比值的升高, 安氏伪镖水蚤的生长速率从(0.067± 0.001)/d减缓到(0.056±0.001)/d, 同时, 高氮条件下, 幼体分别发育5d和18d后进入桡足幼体期和成体期, 氮添加实验组与之相仿, 低氮条件下则推迟到第8天进入桡足幼体期。随着藻细胞C/N比值的升高, 安氏伪镖水蚤各个时期个体的氮含量显著降低, 存活率也随之降低。处于高氮或氮添加条件下的无节幼体、桡足幼体和成体的氮摄食率[(0.53—0.95)×10?4mgN/(ind·h)]均比低氮条件[(0.32—0.6)× 10?4mgN/(ind·h)]要高, 但仅成体的碳摄食率在高氮条件下达(8.11±1.21)×10?4mgC/(ind·h), 明显高于低碳条件下的(7.54±0.35)mgC/(ind·h)。结果表明: 氮缺乏对安氏伪镖水蚤的生长发育和摄食等均有明显的抑制作用。氮添加条件下的饵料仅部分逆转这种抑制作用, 暗示安氏伪镖水蚤的生长和摄食除受氮元素的直接限制外, 还可能由于饵料在低氮时缺乏某些化合物而受限。 |
| 关键词: 桡足类, 生长, 摄食, 氮 |
| DOI：10.11693/hyhz201203031031 |
| 分类号: |
| 基金项目:国家重点基础研究发展计划(973)项目, 2011CB403603 号; 国家自然科学青年基金项目, 40906068 号; 广东省自然科学基金项目, 9451063201002243号; 中央高校基本科研业务费专项资金, 21611427号 |
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| GROWTH AND INGESTION OF PSEUDODIAPTOMUS ANNANDALEI UNDER DIFFERENT NITROGEN CONDITIONS |
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HE Xue-Jia, ZHANG Jun-Gang, SHI Chen, Lü Bo-Dong
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Research Center for Harmful Algae and Marine Biology, Jinan University
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| Abstract: |
| Fed on cells of Tetraselmis subcordiformis with high nitrogen content (HN, molar C/N=8.5), low nitrogen content (LN, C/N=12.7) and with short-term addition of nitrogen after culture in f/2 medium without N (AN, C/N=9.6), the Growth, development and ingestion of Pseudodiaptomus annandalei were investigated. With the increase of C/N of algal cells, growth rate of P. annandalei decreased from (0.067±0.001) to (0.056±0.001) d?1. Nauplii entered into copedites or maturation on Day 5 or 18 after birth under HN condition. Under LN condition, they developed into copedites on Day 8. N contents of nauplii, copedites and adults of P. annandalei decreased with the increase of C/N of algal cells. So did the survivorship. P. annandalei of all three life stages under HN or AN conditions had higher ingestion rates in terms of N [(0.53—0.95)×10?4mgN/(ind·h)] than those under LN condition [(0.32—0.6)×10?4mgN/(ind·h)]. However, only adults un-der HN had higher C ingestion rate than their counterparts under LN condition [(8.11±1.21)×10?4vs. (7.54±0.35)mgC/(ind·h)]. N deficiency significantly suppressed the growth, development and ingestion rate in P. annandalei. The short-term addition of N partly reversed the suppression by LN. |
| Key words: Copepod, Growth, Ingestion rate, Nitrogen |
