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大菱鲆(Scophthalmus maximus)耐高温品系选育及耐温性能评估
马爱军,黄智慧,王新安,郭 黎,雷霁霖,杨 志,曲江波
1.中国水产科学研究院黄海水产研究所 青岛市海水鱼类种子工程与生物技术重点实验室 农业部海洋渔业可持续发展重点实验室;2.烟台市开发区天源水产有限公司
摘要:
采用家系选育方法, 进行了大菱鲆耐高温性状选育研究, 对两代家系耐温性能进行测定, 并利用bCOX 回归分析了各家系的耐温性优势比。结果表明: 各家系耐温性差异达到了显著水平(P<0.05), 并且耐高温能力的优势比也存在显著差异, 这反映出在大菱鲆耐高温选育中家系选择的高效性; 经过一代选育, 获得4个耐高温家系, 并据此构建选育二代家系。耐温性能评估, 选育二代比一代有显著提高, 在27℃高温条件下, 选育一代的平均死亡率为49%, 选育二代的平均死亡率为10.26%; 28℃条件下, 选育二代耐高温子代的平均死亡率为32.83%, 对照组的平均死亡率为88.9%; 经过筛选后, 选育二代的耐温性比选育一代提高了1—2℃左右。
关键词:  大菱鲆, 耐温性, 家系, 优势比, 选育
DOI:10.11693/hyhz201204017017
分类号:
基金项目:现代农业产业技术体系建设专项资金(CARS-50-G01); 国家863计划(2012AA10A408-8)
THE SELECTIVE BREEDING OF THERMAL TOLERANCE FAMILY AND APPRAISAL OF PERFORMANCE IN TURBOT SCOPHTHALMUS MAXIMUS
MA Ai-Jun1,2,3, HUANG Zhi-Hui1,2,3, WANG Xin-An1,2,3, GUO Li1,2,3, LEI Ji-Lin1,2,3, YANG Zhi4, QU Jiang-Bo4
1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;2.Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology;3.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, P. R. China;4.Yantai Tianyuan Aquatic Limited Corporation
Abstract:
In order to carry out the breeding program which about thermal tolerance traits of turbot Scophthalmus maximus L., a large-scale family construction was carry out, and detected the tolerance performance of two generation, and calculate the odd ratio of tolerance by bCOX regression analysis. The results demonstrated that the tolerance and it odd ratio of every family have been at a significance different level (P<0.05), it reflected that the high performance of family breeding method. After the first filial generation breeding, we get four tolerance family, and constructed the second filial generation, to evaluate the thermal tolerance, the result suggested that mortality rate of F2 family just 10.25%, and F1family was 49%, the F2family tolerance was higher than F1, when they in the 27℃condition. Then in the 28℃ condition, F2family the mortality rate just 32.83%, and the mortality ofcontrol group was 88.9%. According to these analysis, we judged that the tolerance of F2 family have increased 1—2℃.
Key words:  Turbot Scophthalmus maximus, Thermal tolerance, Family, Odds ratio, Selective breeding
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