| 摘要: |
| 利用PCR扩增技术, 从泥蚶的cDNA文库中克隆出了ATP合酶F1-β亚基基因。FOF1-ATP合酶是生物体能量代谢的重要合成酶, ATP 合酶F1-β亚基是其重要组成部分。序列分析结果表明, 泥蚶的ATP合酶F1-β亚基全长1944bp, 包括5′端非翻译区(5′-UTR)序列246bp, 3′端非翻译区(3′-UTR)序列126bp和1572bp的开放阅读框序列(ORF), 编码523个氨基酸, 预测分子量大小和等电点分别为54.13kDa和4.73。在重金属镉、铜、铅胁迫下, 检测ATP合酶F1-β亚基和精氨酸激酶基因在转录水平的调控变化。 |
| 关键词: 泥蚶, 重金属, ATP 合酶F1-β亚基, 精氨酸激酶, 荧光定量PCR |
| DOI：10.11693/hyhz201205007007 |
| 分类号: |
| 基金项目:国家自然科学基金项目, 30771665 号 |
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| THE STUDY OF HEAVY METAL STRESS EFFECTS ON THE TRANSCRIPTIONAL LEVEL OF ENERGY METABOLISM ENZYMES OF TEGILLARCA GRANOSA |
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ZHANG Chun-Dan1, ZHOU Jun1, LI Ye1, LI Cheng-Hua1, SU Xiu-Rong1, LI Tai-Wu1,2
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1.School of Marine Sciences, Ningbo University;2.Ningbo City College of Vocational Technology
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| Abstract: |
| By the PCR method, the full length ATP synthase F1-βsubunit gene was cloned from cDNA library of Tegillarca granosa. FOF1-ATP synthase is the crucial enzyme in energy metabolism and βsubunit is the important component of FOF1-ATP synthase. Sequence analysis indicated that total of the gene contain 1944bp, including a 5′-terminal untranslated region (UTR) of 246bp, a 3′-UTR of 126bp and a 1572bp open reading fragment (ORF), encoding 523 amino acid residues, calculated molecular weight and predicted pI were 54.13kDa and 4.73, respectively. After exposed to cadmium, copper and lead, the transcriptional levels of ATP synthase F1-βsubunit and arginine kinase gene were measured. |
| Key words: Tegillarca granosa, Heavy metal, ATP synthase F1-βsubunit, Arginine kinase, Fluorescence quantitative PCR |
