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| 泥蚶(Tegillarca granosa)遗传连锁图谱的构建 |
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刘 博1,2, 滕爽爽1,2, 柴雪良1,2, 邵艳卿1,2, 肖国强1,2, 张炯明1,2, 方 军1,2
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1.浙江省海洋水产养殖研究所;2.浙江省近岸水域生物资源开发与保护重点实验室
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| 摘要: |
| 采用AFLP标记技术结合“拟测交”策略, 对泥蚶父、母本和109个F1全同胞个体进行了遗传连锁的分析研究, 构建了泥蚶的遗传连锁图谱。结果表明, 在638个分离标记中, 符合1 : 1孟德尔分离类型位点的母本和父本分离标记分别为235和259个; 3 : 1孟德尔分离类型位点59个。雌性图谱包括21个连锁群, 标记间平均间隔为8.12cM, 图谱共覆盖1737.8cM, 覆盖率为83.1%; 雄性图谱包括20个连锁群, 标记间平均间隔为5.81cM, 图谱共覆盖1388.2cM, 覆盖率为84.7%。 |
| 关键词: 泥蚶, AFLP, 遗传连锁图谱, 拟测交策略 |
| DOI：10.11693/hyhz201205016016 |
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| 基金项目:国家高技术研究发展计划(863 计划), 2012AA10A410-1 号; 浙江省自然科学基金项目, Y3110003; 浙江省科研院所专项, 2009F20010号; 温州市科技兴海项目, S20080019 号 |
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| CONSTRUCTION OF GENETIC LINKAGE MAP OF TEGILLARCA GRANOSA LINNAEUS |
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LIU Bo1,2, TENG Shuang-Shuang1,2, CHAI Xue-Liang1,2, SHAO Yan-Qing1,2, XIAO Guo-Qiang1,2, ZHANG Jiong-Ming1,2, FANG Jun1,2
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1.Zhejiang Mariculture Research Institute;2.Zhejiang Key Laboratory of Exploitation and Reservation of Coastal Bio-Resource
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| Abstract: |
| Based on segregation of amplified fragment length polymorphism (AFLP) markers, the first genetic linkage map of Tegillarca granosa Linnaeus was constructed using the “two-way pseudo-testcross” strategy. A segregation population (F1) including 109 progenies (full-sib family) from the cross between two wild populations of T. granosa from Jiangsu and Yueqing was obtained. The parents and the F1 progeny were used as the segregation population. A set of 66-pair primer combination were used to analyze the parents and the 109 progenies of the mapping family. A total of 638 segregation sites were identified. Among them, 494 (235 for female and 259 for male) fitted in the expected 1 : 1 Mendelian segregation ratio and 59 fitted in a 3 : 1 Mendelian ration. The female linkage maps included 21 linkage groups, spanning 1737.8cM with the average marker density of 8.12cM, and the observed coverage was 83.1%. The male linkage maps included 20 linkage groups, spanning 1388.2cM with the average marker density of 5.81cM, and the observed coverage was 84.7%. |
| Key words: Tegillarca granosa, AFLP, Genetic linkage map, Double pseudo-testcross strategy |